文献类型：期刊文献

英文题名：A sequence-activated near-infrared fluorescence probe for precisely tracking in vivo senescence

作者：Nong, Jiahuan[1];Li, Pengcheng[1];Zhu, Mingming[2];Li, Yifeng[3];Wang, Changsheng[4];Zhang, Yutao[1];Yan, Chenxu[1];Guo, Zhiqian[1,5]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn, Inst Fine Chem,Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Inflammatory Bowel Dis Res Ctr, Shanghai Inst Digest Dis,Div Gastroenterol & Hepat, Renji Hosp,Sch Med,NHC Key Lab Digest Dis, Shanghai, Peoples R China;[3]East China Normal Univ, High Sch 2, Shanghai 201203, Peoples R China;[4]Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Infect, Shanghai, Peoples R China;[5]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：16

期号：38

起止页码：17658

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20254119290435);WOS:【SCI-EXPANDED(收录号:WOS:001562924000001)】；

基金：This work was supported by NSFC/China (32394001, 22225805, 32121005, and 22378122), Shanghai Science and Technology Innovation Action Plan (No. 23J21901600), Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28), Shanghai Pujiang Program (22PJ1411800), and Science and Technology Commission of Shanghai Municipality (24DX1400200).

语种：英文

外文关键词：Cells - Cytology

摘要：Real-time monitoring of senescent cells is of great significance for understanding and intervening in aging. Since overexpression of endogenous beta-galactosidase (beta-gal) is not unique to senescent cells, probes relying solely on beta-gal activity could yield inaccurate senescent cell detection. Herein, we designed a dual-mode sequential response AND logic NIR probe MFB-beta gal, which contains a beta-gal-cleavable unit and a morpholine unit, serving as an enzymatic activity trigger and a lysosomal targeting moiety, respectively. MFB-OH is generated in situ after reaction with beta-gal, which can detect the alkalinization of lysosomes by emission intensity in senescent cells. This probe has been successfully used to distinguish between SKOV-3 and senescent cells and applied to in vivo visualization of beta-gal activity in a mouse model, providing a new strategy for the accurate detection of cellular senescence.