文献类型：期刊文献

英文题名：Enantioselective molecular imprinting polymer coated QCM for the recognition of l-tryptophan

作者：Liu, Feng[1,2]; Liu, Xiao[1,3]; Ng, Siu-Choon[1]; Chan, Hardy Sze-On[1,4,5]

机构：[1] Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; [2] Institute of Material Science and Technology, East China University of Science and Technology; [3] Department of Chemistry, National University of Singapore; [4] Department of Chemistry; [5] Faculty of Science, National University of Singapore

年份：2006

卷号：113

期号：1

起止页码：234

外文期刊名：Sensors and Actuators, B: Chemical

收录：EI(收录号：2005529620997)

语种：英文

外文关键词：Atomic force microscopy - Biomimetic materials - Coatings - Crosslinking - Crystals - Polymers - Synthesis (chemical) - Thin films

摘要：A new quartz crystal microbalance (QCM) sensor that provides enantioselectivity to tryptophan enantiomers, with a high selectivity and sensitivity, was fabricated by the use of the molecularly imprinted polymers (MIPs) as the artificial biomimetic recognition material. Non-covalent molecular imprinting polymers were synthesized using acrylamide (AM) and trimethylolpropane trimethacrylate (TRIM) as a functional monomer and a cross-linking agent, respectively. The preparation of the thin permeable film coatings on QCM surface is described as well as the results and discussion on the sensitivity and selectivity of the coatings to tryptophan enatiomers under different conditions. The sensor was able to discriminate between the l- and d-tryptophan enantiomers in citric acid buffer solutions owing to the enantioselectivity of the imprinted sites. The detection limit is 8.8 μM. The influence of the cross-linking agent concentration on the sensitivity and selectivity of the fabricated polymer films was investigated and optimized. The enantionmeric composition of l- and d-tryptophan enantiomer mixtures can be determined by measuring the frequency shifts of the sample. The characteristics of the thiol monolayer and MIP film on the surface of the QCM were studied by atomic force microscopy (AFM). ? 2005 Elsevier B.V. All rights reserved.