文献类型：期刊文献

英文题名：Metagenomics and metatranscriptomics uncover the microbial community associated with high S0 production in a denitrifying desulfurization granular sludge reactor

作者：Li, Wei[1,2,3];Gao, Jian[1,2];Zhuang, Jin-long[1,2];Yao, Gen-ji[1,2];Zhang, Xu[1,2];Liu, Yong-di[1,2];Liu, Qi-kai[4];Shapleigh, James P.[5];Ma, Liang[1,2]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Resources & Environm Engn, Natl Engn Lab Ind Wastewater Treatment, Shanghai, Peoples R China;[3]Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing, Peoples R China;[4]Nishihara Environm Engn Shanghai Co Ltd, Shanghai, Peoples R China;[5]Cornell Univ, Dept Microbiol, Ithaca, NY USA

年份：2021

卷号：203

外文期刊名：WATER RESEARCH

收录：;EI(收录号：20213210750908);WOS:【SCI-EXPANDED(收录号:WOS:000697762400002)】；

基金：This work was supported by National Key Research and Develop-ment Program of China (2019YFC0408202) , National Natural Science Foundation of China (51808223) , Shanghai Educational Development Foundation (18CG34) , Shanghai Rising-Star Program (20QC1400500) and State Key Laboratory of Pollution Control and Resource Reuse (PCRRF20024) .

语种：英文

外文关键词：Denitrifying sulfide oxidation; S-0 production; Microflora; Meta-omics

摘要：The denitrification desulfurization process is a promising technology for elemental sulfur (S-0) production from sulfide containing wastewater. However, the microbial community associated with high S-0 production still is not well studied. This study describes an efficient denitrification S-0 production bioreactor based on inoculation with anaerobic granular sludge. At an optimal S/N molar ratio of 7:2, 80 % of the influent sulfide was transformed to high quality elemental sulfur with a purity of 92.5% while the total inorganic nitrogen removal efficiency was stable at similar to 80%. Metatranscriptomic analysis found that community expression of the gene encoding the sulfide-quinone reductase (SQR) was 10-fold greater than that of the flavocytochrome-c sulfide dehydrogenase subunit B (fccB). Moreover, the expression level of SQR was also significantly higher than the Dsr gene encoding for dissimilatory sulfate reductase, which encodes a critical S-0 oxidation enzyme. Metagenomic binning analysis confirmed that sulfide-oxidizing bacteria (SOB) utilizing SQR were common in the community and most likely accounted for high S-0 production. An unexpected enrichment in methanogens and high expression activity of bacteria carrying out Stickland fermentation as well as in other bacteria with reduced genomes indicated a complex community supporting stable sulfide oxidation to S-0, likely aiding in performance stability. This study establishes this treatment approach as an alternative biotechnology for sulfide containing wastewater treatment and sheds light on the microbial interactions associated with high S-0 production.