文献类型：期刊文献

中文题名：Regulating the bioactivity of non-glycosylated recombinant human bone morphogenetic protein-2 to enhance bone regeneration

作者：Yuanman Yu[1,2,3];Rui Chen[1,2];Xinye Chen[1,3];Jing Wang[1,2,3];Changsheng Liu[2,3]

机构：[1]The State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,PR China;[2]Key Laboratory for Ultrafine Materials of Ministry of Education,Engineering Research Center for Biomedical Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai,200237,PR China;[3]Frontiers Science Center for Materiobiology and Dynamic Chemistry,East China University of Science and Technology,Shanghai,200237,PR China

年份：2024

期号：8

起止页码：169

中文期刊名：Bioactive Materials

外文期刊名：生物活性材料（英文）

收录：Scopus;CSCD:【CSCD2023_2024】；PubMed;

基金：supported by the Basic Science Center Program of National Natural Science Foundation of China(No.T2288102);the Key Program of the National Natural Science Foundation of China(No.32230059);the National Natural Science Foundation of China(No.32101086);the National Postdoctoral Program for Innovative Talents(BX2021101);the China Postdoctoral Science Foundation Funded Project(2021M701192);Fundamental Research Funds for the Central Universities(JKD01221507);the Foundation of Frontiers Science Center for Materiobiology and Dynamic Chemistry(JKVD1211002).

语种：英文

中文关键词：Recombinant human bone morphogenetic;protein-2;Osteogenic activity;Protein stability;Sulfated polysaccharide

摘要：Recombinant human bone morphogenetic protein-2(rhBMP-2)is the predominant growth factor that effectively induces osteogenic differentiation in orthopedic procedures.However,the bioactivity and stability of rhBMP-2 are intrinsically associated with its sequence,structure,and storage conditions.In this study,we successfully determined the amino acid sequence and protein secondary structure model of non-glycosylated rhBMP-2 expressed by an E.coli expression system through X-ray crystal structure analysis.Furthermore,we observed that acidic storage conditions enhanced the proliferative and osteoinductive activity of rhBMP-2.Although the osteogenic activity of non-glycosylated rhBMP-2 is relatively weaker compared to glycosylated rhBMP-2;however,this discrepancy can be mitigated by incorporating exogenous chaperone molecules.Overall,such information is crucial for rationalizing the design of stabilization methods and enhancing the bioactivity of rhBMP-2,which may also be applicable to other growth factors.