文献类型：期刊文献

英文题名：Exploration of Glyoxals' Level Changes in Endoplasmic Reticulum During Ferroptosis by a Photocaged Fluorescent Probe

作者：Xing, Wanjin[1]; Zhang, Yirong[1]; Ma, Huijuan[1]; Shen, Shuran[1]; Wang, Sinan[2]; Xu, Huan[3]; Wang, Wei[4]; Lou, Kaiyan[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, Shanghai Key Laboratory of New Drug Design, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science & Technology, 130 Meilong Road, Shanghai, 200237, China; [2] School of Biomedical Engineering, State Key Laboratory of Advanced Medical Materials and Devices, ShanghaiTech University, 393 Huaxia Road, Shanghai, 201210, China; [3] School of Public Health, Anhui University of Science and Technology, Anhui Province, Hefei, 231131, China; [4] Department of Pharmacology and Toxicology, BIO5 Institute, University of Arizona, Tucson, AZ, 85721-0207, United States

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240269537)

语种：英文

外文关键词：Cell death - Fluorescence - Fluorescence spectroscopy - Quenching

摘要：Both ferroptosis and elevated reactive dicarbonyl species (e.g. methylglyoxal and glyoxal, GOS) are associated with endoplasmic reticulum (ER) stress, but their correlations, in particular, how GOS level changes in ER during ferroptosis remains unclear. In this work, a new photoactivatable ER-targeting GOS probe Photo-ER-GOS was designed and synthesized to detect GOS level changes in ER during ferroptosis. The probe contains a photolabile nitrobenzyloxymethyl group functioned both as the reactivity blocking group and the fluorescence quenching group and linked to the guanidino group of NAP-DCP-3, the ER-targeting GOS selective fluorescent probe previously developed in our lab. Cells preincubated with Photo-ER-GOS for 2 h to ensure its precise translocation to ER were then irradiated with 395 nm LED light to release the active probe NAP-DCP-3 for GOS detection in ER. The in situ photodecaging strategy avoids potential interferences from the active probe NAP-DCP-3 reacting with GOS not relevant to ER. Much higher ER GOS level increase was found in ER during erastin-induced ferroptosis than in apoptosis by in situ photodecaging of Photo-ER-GOS, suggesting that upregulated ER GOS levels may be a previously overlooked characteristic of ferroptosis. Erastin-induced GOS level increase was also confirmed in zebrafish by the Photo-ER-GOS. ? 2024, The Authors. All rights reserved.