文献类型：期刊文献

英文题名：Characterization of a novel nitrilase, BGC4, from Paraburkholderia graminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles

作者：Fan, Haiyang[1];Chen, Lifeng[1];Sun, Huihui[2];Wang, Hualei[1];Liu, Qinghai[1];Ren, Yuhong[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Fishery Sci Qingdao, Yellow Sea Fisheries Res Inst, Qingdao Shi 266071, Shandong, Peoples R China

年份：2017

卷号：39

期号：11

起止页码：1725

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20173104011731);WOS:【SCI-EXPANDED(收录号:WOS:000412733200015)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 21406068/B060804), the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Biodegradation; Nitrilase; Paraburkholderia graminis; Wide-spectrum substrate specificity

摘要：To investigate the biodegradation of nitriles via the nitrilase-mediated pathway. A novel nitrilase, BGC4, was identified from proteobacteria Paraburkholderia graminis CD41M and its potential for use in biodegradation of toxic nitriles in industrial effluents was studied. BGC4 was overexpressed in Escherichia coli BL21 (DE3), the recombinant protein was purified and its enzymatic properties analysed. Maximum activity of BGC4 nitrilase was at 30 A degrees C and pH 7.6. BGC4 has a broad substrate activity towards aliphatic, heterocyclic, and aromatic nitriles, as well as arylacetonitriles. Iminodiacetonitrile, an aliphatic nitrile, was the optimal substrate but comparable activities were also observed with phenylacetonitrile and indole-3-acetonitrile. BGC4-expressing cells degraded industrial nitriles, such as acrylonitrile, adiponitrile, benzonitrile, mandelonitrile, and 3-cyanopyridine, showing good tolerance and conversion rates. BGC4 nitrilase has wide-spectrum substrate specificity and is suitable for efficient biodegradation of toxic nitriles.