文献类型：期刊文献

中文题名：钛金属表面骨诱导型可降解壳聚糖涂层的构建及生物活性调控

英文题名：Construction of Osteoinductive and Biodegradable Chitosan-Coating on Titanium Surface and Regulation of Biological Activities

作者：李海霞[1];何宏燕[1];董秀琳[1];常铃雪[1];刘昌胜[1]

机构：[1]华东理工大学材料科学与工程学院教育部医用生物材料工程研究中心

年份：2020

卷号：33

期号：1

起止页码：39

中文期刊名：功能高分子学报

外文期刊名：Journal of Functional Polymers

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金面上项目（31570971）;上海市国际科技合作项目（18520710100）;中国科学院-威高研究发展计划（攻关项目[2017]005号）

语种：中文

中文关键词：钛金属;静电喷涂;rhBMP-2;壳聚糖;阿仑膦酸钠

外文关键词：titanium;electrostatic spraying;rhBMP-2;chitosan;alendronate

摘要：纯钛片(Ti)首先通过喷砂酸蚀(SLA)形成SLA-Ti,然后经阿仑膦酸钠(ALN)亲水处理后形成ALN-SLA-Ti,最后以壳聚糖(CS)为涂层材料,通过静电喷涂(ES)将重组人骨形态发生蛋白-2(rhBMP-2)快速、有效地载入钛片表面,构建载有活性因子的CS涂层来提高钛片表面的生物活性,进而促进钛片表面的成骨能力。利用扫描电子显微镜、激光共聚焦显微镜等对钛片表面形貌、细胞增殖及成骨分化能力进行表征。结果表明:构建的CS涂层具有多级孔洞结构,亲水且可降解;固载的蛋白质持续可控释放;载有rhBMP-2 CS涂层有利于细胞的黏附和增殖,明显促进细胞成骨分化。
In order to improve the biological activity and promote the osteogenic differentiation capability of the implanted metallic materials,SLA-Ti was prepared from pure titanium(Ti)through sandblasting and acid etching(SLA).Then,ALNSLA-Ti was formed by hydrophilic treatment on Ti with alendronate sodium(ALN).Using chitosan(CS)as the main material for the coating,ALN-SLA-Ti surface was coated with CS and immobilized protein by electrostatic spraying(ES).Human bone morphogenetic protein-2(rhBMP-2)was rapidly and efficiently loaded onto the surface of ALN-SLA-Ti.Coatings loaded with active factors were constructed to enhance the biological activity of titanium surfaces.The morphology of the titanium surface was observed by scanning electron microscope.Bovine serum albumin(BSA)was used as a model protein to evaluate the release behavior in vitro.The adhesion and proliferation of cells on the surface of titanium was observed by laser confocal microscopy.Result showed that ALN-SLA-Ti formed a microsphere structure after 5 min of ES.The treated surface had multi-level pore structures.Immobilized protein rhBMP-2 was continuously released in a controlled manner.CS coating with rhBMP-2 was conducive to cell adhesion and proliferation,and significantly promote osteogenic differentiation of cells.