文献类型：期刊文献

英文题名：New Polyketide Congeners with Antibacterial Activities from an Endophytic Fungus Stemphylium globuliferum 17035 (China General Microbiological Culture Collection Center No. 40666)

作者：Li, Yingying[1];Zhu, Guoliang[1];Wang, Jing[1];Yu, Junjie[1];Ye, Ke[1];Xing, Cuiping[1];Ren, Biao[2,3];Zhu, Bin[4];Chen, Simin[1];Lai, Lijun[1];Li, Yue[1];Hsiang, Tom[5];Zhang, Lixin[1];Liu, Xueting[1];Zhang, Jingyu[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China;[3]Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ,Lab Pharmaceut Crystal Engn & Technol, Shanghai 200237, Peoples R China;[5]Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

年份：2024

卷号：10

期号：11

外文期刊名：JOURNAL OF FUNGI

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001365357400001)】；

基金：This work was supported by the National Key Research and Development Program of China (2020YFA0907200 and 2020YFA0907800), the National Natural Science Foundation of China (32121005 and 21877038), Shanghai Rising-Star Program (20QA1402800), Open Project Funding of the State Key Laboratory of Bioreactor Engineering, the 111 Project (B18022), and the Fundamental Research Funds for the Central Universities. Discovery and isolation of Stemphylium globuliferum 17035 was partially supported by the Natural Sciences and Engineering Research Council of Canada funding to T. Hsiang.

语种：英文

外文关键词：endophytic fungus; polyketides; Stemphylium globuliferum; antibacterial; anticancer; OSMAC strategy

摘要：Four new polyketides, heterocornol Y (1), stemphyindan (2), pestalospirane C (3), and stemphyspyrane (4), along with five known ones (5-9) were isolated from the endophytic fungus Stemphylium globuliferum 17035 (SG17035) based on the One Strain Many Compounds (OSMAC) strategy allied with an LC-MS approach. These structures were elucidated through extensive spectroscopic analyses, single-crystal X-ray diffraction, and 13C NMR-DP4 analysis. Pestalospirane C (3) and stemphyspyrane (4) featured unprecedented spiroketal skeletons. In addition, the putative biosynthetic logic for compounds 1-4 was proposed. Antibacterial and cytotoxic activities of compounds 1-9 were evaluated. Stemphyspyrane (4) displayed promising antibacterial activity against different pathogens, especially against Staphylococcus aureus, Porphyromonas gingivalis, and methicillin-resistant Staphylococcus aureus (MRSA) with MIC values of 3.125 mu M, 6.25 mu M, and 12.5 mu M, respectively. It is promising as an antibacterial agent for further optimization.