文献类型：期刊文献

英文题名：Investigation of compatible solutes synthesis and transport of Virgibacillus halodenitrificans PDB-F2 with complete genome analysis

作者：Zhou, Yang[1];Sun, Zhi-Yuan[1];Li, Hui[1];Qian, Chan-Juan[1];Wu, Xiao[1];Tang, Hong-Zhi[2,3];Ali, Fawad[2,3];Liu, Yong-Di[1]

机构：[1]East China Univ Sci & Technol, Sch Resource & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China

年份：2017

卷号：122

起止页码：165

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20172103695248);WOS:【SCI-EXPANDED(收录号:WOS:000403858400018)】；

基金：This work was supported jointly by the National Natural Science Foundation of China (41273109, 51378208 and 51578240), the National Key Research and Development Plan (2016YFC0206200), the Fok Ying Tung Education Foundation (141077), the Innovation Program of the Shanghai Municipal Education Commission (14ZZ059) and the Fundamental Research Funds for the Central Universities (WB1516015 and WB1616012). We also would like to thank the anonymous referees for their helpful comments on this paper.

语种：英文

外文关键词：Virgibacillus halodenitrificans PDB-F2; Compatible solutes; Genome sequencing; Genome analyzing; Synthesis genes; Transport genes

摘要：The salt-tolerant mechanism of compatible solutes in the different microorganisms is always a research hotspot, which can help us understand how organism endures the salty environment. Virgibacillus halodenitrificans PDB-F2 could survive in high salinity and degrade phenol, which is a good candidate for wastewater treatment. This study investigated the salt-tolerant mechanism of compatible solutes of this strain and got an insight into its genetic basis through genome sequencing and analyzing. The results found that Virgibacillus halodenitrificans PDB-F2 endured 12% (w/v) NaCl condition by synthesizing or uptaking ectoine, hydroxyectoine, trehalose, glutamic acid and betaine. Osmoprotective effects of exogenous compatible solutes on this strain were hydroxyectoine > ectoine > L-proline > trehalose > glutamate acid > betaine. Under osmotic shock, the strain had a higher preference for hydroxyectoine than ectoine, and the ectoine transport was stimulated at both levels of transport activity and transcription. The sequencing and analyzing of strain genome showed that this strain contained a circular chromosome (3,869,935 bp) and one plasmid (47,824 bp), revealing the genes related with synthesis and transport of above compatible solutes. This study provided further information on the understanding of salt-tolerant mechanism of Virgibacillus halodenitrificans PDB-F2 by compatible solutes. (C) 2017 Elsevier Ltd. All rights reserved.