文献类型：期刊文献

英文题名：Biofilm Polysaccharide Display Platform: A Natural, Renewable, and Biocompatible Material for Improved Lipase Performance

作者：Dong, Hao[1];Zhang, Wenxue[1];Wang, Yibing[1];Liu, Dong[2];Wang, Ping[3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn,Biomed Nanotechnol, Shanghai 200237, Peoples R China;[2]Nanjing Tech Univ, Natl Engn Res Ctr Biotechnol, Coll Biotechnol & Pharmaceut Engn, Nanjing 211816, Peoples R China;[3]Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA

年份：2020

卷号：68

期号：5

起止页码：1373

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20200908217896);WOS:【SCI-EXPANDED(收录号:WOS:000512222600021)】；

基金：This work was sponsored by the National Natural Science Foundation of China (21636003), the Natural Science Foundation of Shanghai (19ZR1412400), and the Fundamental Research Funds for the Central Universities (22221818014). We would like to thank Editage (www.editage.cn) for English language editing.

语种：英文

外文关键词：immobilization; biofilm; Clostridium acetobutylicum; lipase; cinnamyl acetate

摘要：Most of the microorganisms can form biofilms, which makes biofilms an abundant bioresource to be exploited. Due to the limitations of the application of current immobilization methods for biofilms, we developed an immobilization method called the biofilm polysaccharide display (BPD) strategy while maintaining the native biofilm structure and catalytic microenvironment of Clostridium acetobutylicum B3. Lipase Lip181 showed significant improvements in stability after chemical immobilization. For example, immobilized Lip181 retained 74.23% of its original activity after incubation for 14 days, while free Lip181 was totally deactivated. In addition, immobilized Lip181 maintained high residual activity (pH 5.0-11.0), which showed improved resistance to pH changes. Notably, this method did not decrease but slightly increased the relative activity of Lip181 from 6.39 to 6.78 U/mg. Immobilized Lip181 was used to prepare cinnamyl acetate, and it showed a maximum yield of 85.09%. Overall, this biofilm immobilization method may promote the development of biocatalytic and biofilm materials.