文献类型：期刊文献

英文题名：Dynamic Visualization of Endoplasmic Reticulum Stress in Living Cells via a Two-Stage Cascade Recognition Process

作者：Wu, Man-Sha[1,2];Zhou, Ze-Rui[1,2];Wang, Xiao-Yuan[1,2];Chen, Bin-Bin[1,2];Hafez, Mahmoud Elsayed[1,2,3];Shi, Ji-Fen[1,2];Li, Da-Wei[1,2];Qian, Ruo-Can[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]Beni Suef Univ, Fac Sci, Dept Chem, Bani Suwayf 62511, Egypt

年份：2022

卷号：94

期号：6

起止页码：2882

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20220711620150);WOS:【SCI-EXPANDED(收录号:WOS:000757978100001)】；

基金：This research was supported by the National Natural Science Foundation of China (21977031, 21777041, and 21974046), the Shanghai Science and Technology Committee (19ZR1472300, 19391901700, and 19520744000), and the Fundamental Research Funds for the Central Universities. The authors thank the Research Center of analysis and Test of East China University of Science and Technology for help on confocal FL imaging analysis.

语种：英文

外文关键词：Cell death - Visualization - Biosynthesis - Fluorescence

摘要：The endoplasmic reticulum (ER) is crucial for the regulation of multiple cellular processes, such as cellular responses to stress and protein synthesis, folding, and posttranslational modification. Nevertheless, monitoring ER physiological activity remains challenging due to the lack of powerful detection methods. Herein, we built a two-stage cascade recognition process to achieve dynamic visualization of ER stress in living cells based on a fluorescent carbon dot (CD) probe, which is synthesized by a facile one-pot hydrothermal method without additional modification. The fluorescent CD probe enables two-stage cascade ER recognition by first accumulating in the ER as the positively charged and lipophilic surface of the CD probe allows its fast crossing of multiple membrane barriers. Next, the CD probe can specifically anchor on the ER membrane via recognition between boronic acids and o-dihydroxy groups of mannose in the ER lumen. The two-stage cascade recognition process significantly increases the ER affinity of the CD probe, thus allowing the following evaluation of ER stress by tracking autophagy-induced mannose transfer from the ER to the cytoplasm. Thus, the boronic acid-functionalized cationic CD probe represents an attractive tool for targeted ER imaging and dynamic tracking of ER stress in living cells.