文献类型：期刊文献

英文题名：A new ganoderic acid from Ganoderma lucidum mycelia and its stability

作者：Li, Ying-Bo[1];Liu, Ru-Ming[1];Zhong, Jian-Jiang[1,2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China

年份：2013

卷号：84

起止页码：115

外文期刊名：FITOTERAPIA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000316531400018)】；

基金：Financial support from the National Basic Research Program of China (973 program no. 2012CB721006), the National Natural Science Foundation of China (NNSFC project no. 30821005) and the National High Technology R&D Program (863 project no. 2012AA021701) is gratefully acknowledged. The authors also express their appreciation to Dr. Jie-Li Wu (Analytical Center of Shanghai Jiao Tong University) and Dr. Feng Lei (Analytical Center of Shanghai Jiao Tong University) for their kind help in spectrum analysis.

语种：英文

外文关键词：Ganoderma lucidum; Ganoderic acid; Cytotoxicity; Stability

摘要：A new ganoderic acid (GA), 3 alpha,22 beta-diacetoxy-7 alpha-hydroxyl-5 alpha-lanost-8,24E-dien-26-oic acid (1), together with four known compounds GA-Mk (2), -Mc (3), -S (4) and -Mf (5), was isolated and characterized from Ganoderma lucidum mycelia. The structure of compound 1 was elucidated on the basis of interpretation of extensive spectroscopic data (HRMS, IR, UV, 1D and 2D NMR). Due to its apparent degradation during preparation procedures, the stability of compound I was assessed in several solvents in a short-term study that demonstrated the optimal stability in aproptic environment. A possible mechanism of acid-catalyzed degradation of compound 1 in methanol was proposed, consisting of a fast protonation, followed by a committed step of hydroxyl group removal. In addition, all isolated compounds were tested in vitro for their cytotoxic activities against 95D and HeLa tumor cell lines, with IC50 values ranging from14.7 to 38.5 mu M. The results may improve the understanding of chemical stability of GAs and provide valuable information on their separation, analysis and application. (c) 2012 Elsevier B.V. All rights reserved.