文献类型：期刊文献

英文题名：Facile preparation of molybdenum (VI) oxide - Modified graphene oxide nanocomposite for specific enrichment of phosphopeptides

作者：Sun, Haofan[1];Zhang, Quanqing[2];Zhang, Lei[1];Zhang, Weibing[1];Zhang, Lingyi[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Dept Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Fudan Univ, Shanghai 200433, Peoples R China

年份：2017

卷号：1521

起止页码：36

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20173904204949);WOS:【SCI-EXPANDED(收录号:WOS:000413382800005)】；

基金：This research was financially supported by Science Foundation for Young Scientists of China (No. 21105027), support of the National Natural Science Foundation of China (No.21475044), the Key Research Project from the Ministry of Public Security (201202ZDYJ005), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044).

语种：英文

外文关键词：MoO3; Graphene oxide; Nanocomposite; Metal oxide affinity chromatography; Phosphopeptide enrichment

摘要：To promote the development of phosphoproteome analysis in highly selective efficient tracing phosphorylated proteins or peptides, views of researches should not confined with intrinsic materials and their modification. New materials are supposed to be explored for phosphoproteome analysis. In this work, we first introduced Molybdenum (VI) oxide (MoO3) into phosphoproteome, loading on the graphene oxide (GO) nanosheets forming MoO3/GO nanocomposites by a simple two-step strategy. The GO nanosheets offered MoO3 a perfect stable platform for separation and concentration and MoO3 exhibited wonderful property in enriching phosphopeptides with highly selectivity and sensitivity on GO nanosheets. Specifically, the as-synthesized MoO3/GO nanocomposites exhibited excellent specificity (beta-casein: BSA = 1:1000), high detection sensitivity (1 fmol/mL) and well recovery (91.13%) in enriching phosphopeptides by metal oxide affinity chromatography (MOAC). Moreover, the as-synthesized MoO3/GO nanocomposites provided effective enrichment of phosphopeptides from nonfat milk (a total of twelve phosphopeptides signals) and human serum (a total of four endogenous phosphopeptides signals), displaying great biological compatibility, which demonstrated that the MoO3/GO nanocomposites is a promising candidate in selectively identifying and determining low-abundance phosphorylated peptides in biological sample. (C) 2017 Elsevier B.V. All rights reserved.