文献类型：期刊文献

英文题名：Efficient accumulation of high-value bioactive substances by carbon to nitrogen ratio regulation in marine microalgae Porphyridium purpureum

作者：Li, Shaohua[1];Ji, Liang[1];Chen, Cheng[2];Zhao, Shuxin[2];Sun, Meng[2];Gao, Zhengquan[3];Wu, Haizhen[1,2];Fan, Jianhua[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]Shandong Univ Technol, Sch Life Sci, Zibo 255000, Peoples R China

年份：2020

卷号：309

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20201608434964);WOS:【SCI-EXPANDED(收录号:WOS:000569074800022)】；

基金：This work was sponsored by National Key Research and Development Project of China 2019YFA0906300, Natural Science Foundation of Shandong Province ZR2019ZD17, National Natural Science Foundation of China 31872608, Natural Science Foundation of Shanghai 18ZR1410100 and 17ZR1406700, Shanghai Pujiang Program 18PJD008, and Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Porphyridium purpureum; C/N ratio; Phycoerythrin; Exopolysaccharides; Fatty acids

摘要：An efficient biomass and high-value bioactive substances production strategy was developed for unicellular microalgae Porphyridium purpureum. We studied the optimal culture method and metabolites accumulation under different C/N conditions, and further proposed methods to increase the yield under high C/N ratio. The highest biomass reached 16.24 g/L with ASW medium by mixotrophy. High C/N ratio and mediate C/N can significantly promote the synthesis and secretion of polysaccharides, as well as the accumulation of omega-6 PUFAs; however, inhibit the growth, resulting in lower yield. With the significant increase of C/N ratio, protein degradation was accelerated, providing sufficient nitrogen source for efficient accumulation of carbohydrates (1.66 g/L EPS) and PUFAs (231.24 mg/L ARA). Finally, we reduced the growth inhibition, shortened the culture cycle, and doubled the final biomass to 9.34 g/L under nitrogen deficiency condition. Our exploitation of a cost-effective and feasible culture method for red algae is particularly significant.