文献类型：期刊文献

中文题名：氧化葡萄糖酸杆菌实时定量PCR检测方法的建立与应用

英文题名：Establishment and Application of Real-time PCR Assay for Quantification of Gluconobacter oxydans

作者：刘旭[1];魏东芝[1];林金萍[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2011

卷号：42

期号：9

起止页码：651

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：氧化葡萄糖酸杆菌;荧光定量PCR;山梨醇脱氢酶

外文关键词：Gluconobacter oxydans; fluorescence quantitative PCR; sorbitol dehydrogenase

摘要：选取氧化葡萄糖酸杆菌16S rRNA作为看家基因,利用Primer Premier 5 0软件设计特异性引物,以总基因组为模板,建立氧化葡萄糖酸杆菌实时定量PCR方法。选取山梨醇脱氢酶大亚基.sldA和小亚基sldB为研究对象,以看家基因16S rRNA的表达作为内参,测得山梨醇脱氢酶大小亚基的转录水平一致,均为看家基因的68%。
A method using 16S rRNA as the housekeeping gene was established for the detection of Gluconobacter oxydans based on real-time quantitative PCR. Primers were designed by software Primer Premier 5.0 that are specific for the recognition of a conservative region in target gene. Sorbitol dehydrogenase containing two subunits, sldA and sldB, was detected and quantified with the transcriptional level of 16S rRNA. The result showed that the transcriptional level of these subunits were 68 % of that of the housekeeping gene.