文献类型：期刊文献

英文题名：Optimization of polyhydroxyalkanoates fermentations with on-line capacitance measurement

作者：Li, Lan[1];Wang, Ze-Jian[1];Chen, Xue-Jun[2];Chu, Ju[1];Zhuang, Ying-Ping[1];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Grp Tian An Pharmaceut Co Ltd, Zhejiang Pharma, Hangzhou 315000, Zhejiang, Peoples R China

年份：2014

卷号：156

起止页码：216

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20140717311808);WOS:【SCI-EXPANDED(收录号:WOS:000334828500030)】；

基金：This work was financially supported by a Grant from the Major State Basic Research Development Program of China (973 Program), No. 2012CB721006, National High Technology Research and Development Program, No. 2012AA021201, and National Key Technology Support Program, No. 2011BAF02B04. We also thank Tianan Pharmacy Corporation (Zhejiang, China) for donating the industrial strain Ralstonia eutropha TA-032.

语种：英文

外文关键词：On-line capacitance measurement; Viable biomass; PHAs; Optimization

摘要：The aim of this work was to provide an effective methodology for optimization of the polyhydroxyalkanoates (PHAs) fermentation with Ralstonia eutropha by the on-line capacitance measurement. The present study found the capacitance values could reflect variations of microbial morphology and viability. Furthermore, oxygen uptake rate, specific oxygen uptake rate and specific growth rate were measured in real-time and compared with the capacitance value. In addition, a fed-batch control strategy based on the on-line capacitance measurement was proposed to improve the PHAs production by 22%. (C) 2014 Elsevier Ltd. All rights reserved.