文献类型：期刊文献

中文题名：Microfluidic reactor with immobilized enzyme-from construction to applications:A review

作者：Han Zhang[1];Yunpeng Bai[1];Ning Zhu[2];Jianhe Xu[1]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;[2]College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing,211800,China

年份：2021

卷号：34

期号：2

起止页码：136

中文期刊名：Chinese Journal of Chemical Engineering

外文期刊名：中国化学工程学报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；

语种：英文

中文关键词：Microfluidic immobilized enzyme reactor;Immobilization strategies;Biocatalysis;Bioconversion;Bioanalysis

摘要：Microfluidic,as the systems for using microchannel(micron-or sub-micron scale)to process or manipulate microflow,is being widely applied in enzyme biotechnology and biocatalysis.Microfluidic immobilized enzyme reactor(MIER)is a tool with great value for the study of catalytic property and optimal reaction parameter in a flourishing and highly producing manner.In view of its advantages in efficiency,economy,and addressable recognition especially,MIER occupies an important position in the investigation of life science,including molecular biology,bioanalysis and biosensing,biocatalysis etc.Immobilization of enzymes can generally improve their stability,and upon most occasions,the immobilized enzyme is endowed with recyclability.In this review,the enzyme immobilization techniques applied in MIER will be discussed,followed by summarizing the novel developments in the field of MIER for biocatalysis,bioconversion and bioanalysis.The preponderances and deficiencies of the current state-of-the-art preparation ways of MIER are peculiarly discussed.In addition,the prospects of its future study are outlined.