文献类型：期刊文献

英文题名：Dry biorefining maximizes the potentials of simultaneous saccharification and co-fermentation for cellulosic ethanol production

作者：Liu, Gang[1];Zhang, Qiang[1];Li, Hongxing[2];Qureshi, Abdul S.[1];Zhang, Jian[1];Bao, Xiaoming[3];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Qilu Univ Technol, Dept Bioengn, Shandong Prov Key Lab Microbial Engn, Jinan, Shandong, Peoples R China;[3]Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, 27 Shanda Nan Rd, Jinan 250100, Shandong, Peoples R China

年份：2018

卷号：115

期号：1

起止页码：60

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20174804466009);WOS:【SCI-EXPANDED(收录号:WOS:000416114400005)】；

基金：National Basic Research Program of China, Grant number: 2011CB707406; Sunshine New Energy Co., Inner Mongolia, China, Grant number: F200-81510s; Science and Technology Major Project in Shandong Province (Emerging Industries), Grant number: 2015ZDXX0403B02

语种：英文

外文关键词：biodetoxification; cellulosic ethanol; dry acid pretreatment; lignocellulose; simultaneous saccharification and co-fermentation (SSCF); wastewater generation

摘要：Despite the well-recognized merits of simultaneous saccharification and co-fermentation (SSCF) on relieving sugar product inhibition on cellulase activity, a practical concomitance difficulty of xylose with inhibitors in the pretreated lignocellulose feedstock prohibits the essential application of SSCF for cellulosic ethanol fermentation. To maximize the SSCF potentials for cellulosic ethanol production, a dry biorefining approach was proposed starting from dry acid pretreatment, disk milling, and biodetoxification of lignocellulose feedstock. The successful SSCF of the inhibitor free and xylose conserved lignocellulose feedstock after dry biorefining reached a record high ethanol titer at moderate cellulase usage and minimum wastewater generation. For wheat straw, 101.4g/L of ethanol (equivalent to 12.8% in volumetric percentage) was produced with the overall yield of 74.8% from cellulose and xylose, in which the xylose conversion was 73.9%, at the moderate cellulase usage of 15mg protein per gram cellulose. For corn stover, 85.1g/L of ethanol (equivalent to 10.8% in volumetric percentage) is produced with the overall conversion of 84.7% from cellulose and xylose, in which the xylose conversion was 87.7%, at the minimum cellulase usage of 10mg protein per gram cellulose. Most significantly, the SSCF operation achieved the high conversion efficiency by generating the minimum amount of wastewater. Both the fermentation efficiency and the wastewater generation in the current dry biorefining for cellulosic ethanol production are very close to that of corn ethanol production, indicating that the technical gap between cellulosic ethanol and corn ethanol has been gradually filled by the advancing biorefining technology.