文献类型：期刊文献

英文题名：Nanopipette-assisted single cell metabolic glycan labeling

作者：Zhou, Ze-Rui[1];Wang, Xiao-Yuan[1];Lv, Jian[1];Chen, Bin-Bin[1];Tang, Yi-Bin[1];Qian, Ruo-Can[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2019

卷号：9

期号：53

起止页码：30666

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20194207540036);WOS:【SCI-EXPANDED(收录号:WOS:000489652900008)】；

基金：This research was supported by National Natural Science Foundation of China (21605048), National Science and Technology Major Project of China (2018ZX10302205), Natural Science Foundation of Shanghai (19ZR1472300) and Chenguang Program (16CG35).

语种：英文

外文关键词：Electroosmosis - Polysaccharides

摘要：Here, we report a single cell glycan labeling strategy by combining nanoscale intracellular glass electrodes with bioorthogonal reaction. With the tip diameter less than 100 nm, the nanopipette electrode can be spatially controlled to inject artificial monosaccharides into single living cells with minimal invasion. The injection process can be precisely regulated by electroosmotic flow inside the nanopipette, and fluorescence labeling of sialic acid at single cell level is achieved.