文献类型：期刊文献

英文题名：Fluorescent polarity probes for identifying bovine serum albumin: Amplification effect of para-substituted benzene

作者：Bai, Hongyan[1];Qian, Junhong[1];Tian, Haiyu[1];Pan, Wenwen[1];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：103

起止页码：1

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20135017083745);WOS:【SCI-EXPANDED(收录号:WOS:000331781600001)】；

基金：This work was financially supported by National 973 Program (No. 2011CB910403) and the Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University (No. SKLF-KF-201215). Dr. J. Qian thanks the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry. We appreciate Prof. A. M. Brouwer (University of Amsterdam) for his kind help.

语种：英文

外文关键词：Fluorescence probe; BSA; Solvatochromic effect; Coumarin; Polarity; Twisted intramolecular charge transfer

摘要：Fluorescent probes 1-3 with coumarin as the fluorophore were designed and synthesized for the determination of bovine serum albumin (BSA). All three probes exhibited evidently solvatochromic UV-vis and fluorescence spectra. Compound 3 was the most effective towards the solvent's polarity: 155 nm (vs. 60 nm for 1 and 100 nm for 2) red shift in the emission maximum was found as the solvent changing from cyclohexane to phosphate buffer solution. These compounds were applied to detect BSA based on the hypothesis that the polarity of the microenvironment surrounding the probe will undergo significant change when the probe moves from the bulk solution to the hydrophobic domains of BSA. 3 was the most sensitive towards BSA and the detection limit of BSA was 0.6 mu g/mL with 3 as the probe, which ensured the detection of BSA content in fetal bovine serum with good recovery. (C) 2013 Elsevier Ltd. All rights reserved.