文献类型：期刊文献

英文题名：Microfluidic-templated cell-laden microgels fabricated using phototriggered imine-crosslinking as injectable and adaptable granular gels for bone regeneration

作者：An, Chuanfeng[1,2,3,4];Zhou, Renjie[5];Zhang, Haoyue[1];Zhang, Yujie[1];Liu, Weijian[6];Liu, Jia[3,4];Bao, Bingkun[7];Sun, Kai[1];Ren, Changle[6,8];Zhang, Yang[3,4];Lin, Qiuning[7];Zhang, Lijun[9];Cheng, Fang[10];Song, Jiankang;Zhu, Linyong[5,7];Wang, Huanan[1]

机构：[1]Dalian Univ Technol, Sch Bioengn, State Key Lab Fine Chem, Dalian 116023, Peoples R China;[2]Shenzhen Univ, Hlth Sci Ctr, Sch Biomed Engn, Guangdong Key Lab Biomed Measurements & Ultrasound, Shenzhen 518060, Peoples R China;[3]Longgang Dist Peoples Hosp Shenzhen, Cent Lab, Shenzhen 518172, Guangdong, Peoples R China;[4]Chinese Univ HongKong, Affiliated Hosp 2, Shenzhen 518172, Guangdong, Peoples R China;[5]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[6]Dalian Municipal Cent Hosp, Dept Joint Surg, Dalian 116044, Peoples R China;[7]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China;[8]Dalian Univ Technol, Fac Med, Dalian 116023, Peoples R China;[9]Third Peoples Hosp Dalian, Dalian Eye Hosp, Dalian 116024, Peoples R China;[10]Dalian Univ Technol, Sch Chem Engn, Key State Lab Fine Chem, Dalian 116023, Peoples R China

年份：2023

卷号：157

起止页码：91

外文期刊名：ACTA BIOMATERIALIA

收录：;EI(收录号：20225013234260);WOS:【SCI-EXPANDED(收录号:WOS:000931535900001)】；

基金：This work was granted by the National Key Research and Development Program of China (No. 2018YFA0703000, 2019YFA0110500) , the National Natural Science Foundation of China (No. 21773022 , 31870957 , 22022506 , and 81900264) , the Fundamental Research Funds for the Central Universities (No. DUT20YG103 and DUT22LAB601) , Dalian Science and Technology Innovation Foundation (2021JJ13SN49) , the Guangdong Provincial Basic and Applied Basic Research (2019A1515110415) , the Long-gang Medical and Health Science and Technology Project (LGK-CYLWS2020040) , and Shenzhen Basic Research Program general project (JCYJ20190808152211686 and JCYJ20190808120217133) .

语种：英文

外文关键词：Microfluidic droplet; Cell-laden microgels; Granular gel; Bone regeneration; Phototriggered imine-crosslinking

摘要：Injectable granular gels consisting of densely packed microgels serving as scaffolding biomaterial have re-cently shown great potential for applications in tissue regeneration, which allow administration via mini-mally invasive surgery, on-target cargo delivery, and high efficiency in nutrient/waste exchange. However, limitations such as insufficient mechanical strength, structural integrity, and uncontrollable differentiation of the encapsulated cells in the scaffolds hamper their further applications in the biomedical field. Herein, we developed a new class of granular gels via bottom-up assembly of cell-laden microgels via photo -triggered imine-crosslinking (PIC) chemistry based on the microfluidic technique. The particulate nature of the granular gels rendered them with shear-thinning and self-healing behavior, thereby functioning as an injectable and adaptable cellularized scaffold for bone tissue regeneration. Specifically, single cell -laden, monodisperse microgels composed of methacrylate-and o-nitrobenzene-functionalized hyaluronic acid and gelatin were prepared using a high-throughput microfluidic technique with a production rate up to 3.7 x 10 8 microgels/hr, wherein the PIC chemistry alleviated the oxygen inhibition on free-radical polymerization and facilitated enhanced fabrication accuracy, accelerated gelation rate, and improved net-work strength. Further in vitro and in vivo studies demonstrated that the microgels can serve as carriers to support the activity of the encapsulated mesenchymal stem cells; these cell-laden microgels can also be used as cellularized bone fillers to induce the regeneration of bone tissues as evidenced by the in vivo experiment using the rat femoral condyle defect model. In general, these results represent a significant step toward the precise fabrication of engineered tissue mimics with single-cell resolution and high cell -density and can potentially offer a powerful tool for the design and applications of a next generation of tissue engineering strategy.