文献类型：期刊文献

英文题名：Preparation and Mechanism Insight of Biodegradable Kippah Vesicles

作者：Wei, Ping[1];Gu, Nannan[1];Gu, Qianxi[2,3];Jiang, Jinhui[2,3];Chen, Jinghua[1];Du, Jianzhong[2,3,4]

机构：[1]Jiangnan Univ, Sch Life Sci & Hlth Engn, Wuxi 214122, Peoples R China;[2]Tongji Univ, Shanghai Peoples Hosp 4, Translat Res Inst Brain & Brain Intelligence, Sch Med,Dept Gynaecol & Obstet,Shanghai Key Lab An, Shanghai 200434, Peoples R China;[3]Tongji Univ, Sch Mat Sci & Engn, Dept Polymer Mat, Shanghai 201804, Peoples R China;[4]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：21

期号：23

外文期刊名：SMALL

收录：;EI(收录号：20252018446351);WOS:【SCI-EXPANDED(收录号:WOS:001472270300001)】；

基金：This project was supported by the National Natural Science Foundation of China (22305102 and 22335005), Innovation Program of Shanghai Municipal Education Commission (2023ZKZD28), the Natural Science Foundation of Jiangsu Province (BK20221097), and the Fundamental Research Funds for the Central Universities (JUSRP123042).

语种：英文

外文关键词：aggregation-induced emission; kippah vesicles; polypeptides; self-assembly

摘要：Kippah vesicles, fully collapsed polymersomes formed during the self-assembly process, are characterized by a bowl-shaped nanostructure with a large specific surface area, high loading capacity, and an internal void. Current research shows that these structural features have primarily been achieved using non-biodegradable block copolymers, while the fundamental mechanism behind their formation is not well understood. Thus, designing biodegradable kippah vesicles and elucidating their formation mechanism is critical. In this study, a tetraphenylethylene (TPE) moiety - a luminogen with aggregation-induced emission (AIE) properties - is strategically introduced into the block copolymer side chain-, yielding the novel polypeptide poly(ethylene glycol)45-block-poly[(glutamic acid-TPE)26-stat-(glutamic acid)29] [PEG(4)(5)-b-P(GATPE(2)(6)-stat-GA(2)(9))]. This polypeptide could self-assemble into kippah vesicles driven by hydrophobic interactions and hydrogen bonding, as confirmed by Fourier-transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-vis) absorption, photoluminescence spectroscopy, and morphological characterization across different aggregation states. Notably, the intrinsic fluorescence of these kippah vesicles exhibited high cellular internalization efficiency and excellent cytocompatibility, highlighting their potential for biomedical applications such as bioimaging and targeted cellular delivery.