文献类型：期刊文献

英文题名：Determination of phenols with ion chromatography-online electrochemical derivatization based on porous electrode-fluorescence detection

作者：Wu, Shuchao[1,2];Yang, Bingcheng[3];Xi, Lingling[1];Zhu, Yan[1]

机构：[1]Zhejiang Univ, Dept Phys, Hangzhou 310028, Zhejiang, Peoples R China;[2]W Anhui Univ, Dept Biol & Pharmaceut Engn, Luan 237012, Anhui, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2012

卷号：1229

起止页码：288

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20120814791253);WOS:【SCI-EXPANDED(收录号:WOS:000301606600036)】；

基金：This research was financially supported by National Natural Science Foundation of China (Nos. 20775070, 20911140271, J0830413), Zhejiang Provincial Natural Science Foundation of China (Nos. R4080124, Y4090104, Y4090078), National Science Foundation of Anhui Provincial Education Department (Nos. KJ2011B211, 2010SQRL189) and the opening foundation of the State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital of Medical College, Zhejiang University (grant No. 2010KF07). We thank Hu Liu (West Anhui University, China) for his help of English.

语种：英文

外文关键词：Phenols; Ion chromatography; Porous electrode; Fluorescence detection

摘要：The current study describes the determination of phenols using ion chromatography-online electrochemical derivatization-fluorescence detection (IC/ED/FD). Six model phenols including 4-methylphenol (pMP), 2, 4-dimethylphenol (DMP), 4-tert-butylphenol (TBP), 4-hydroxylphenolacetic acid (pHPA), 4-acetamidophenol (pAAP), and phenol (P) were well separated on an anion-exchange column under ion exchange mode using NaOH with small amount of acetonitrile added as eluent. Online electrochemical derivatization performed via a laboratory-made electrolytic cell (EC), consisting of porous titanium electrode and cation-exchange membrane (CEM), allows the oxidation products that are strongly fluorescent to be detected by the fluorescence detector. NaOH eluent used in the present method matches well with the maximal fluorescence intensity obtained at alkaline condition for oxidized phenols, thus the addition of specific buffer solution after oxidation encountered in previous report could be eliminated. This process leads to a simplified procedure. The proposed method was sensitive to the limits of detection in the range of 0.4 mu g/L and 3.8 mu g/L and the limits of quantification between 1.2 mu g/L and 13 mu g/L due to the strong electro-oxidation capacity of porous titanium electrode, as well as the implementation of time-programmed potential over EC. The linear ranges were 2.0-1.0 x 10(4) mu g/L for pAAP and DMP, and 10-1.0 x 10(4) mu g/L for P, pMP, pHPA, and TBP, respectively. The relative standard deviations range from 0.9% to 4.8%. The utilization of the method was demonstrated by the analysis of real samples. The average spiked recoveries of target analytes in pool water were 81.0-118%. (C) 2012 Elsevier B.V. All rights reserved.