文献类型：期刊文献

英文题名：A highly selective and sensitive near-infrared fluorescence probe for arylamine N-acetyltransferase 2 in vitro and in vivo

作者：Wang, Xin[1]; Cui, Lei[1]; Zhou, Nannan[1]; Zhu, Weiping[1]; Wang, Rui[1]; Qian, Xuhong[1]; Xu, Yufang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

年份：2013

卷号：4

期号：7

起止页码：2936

外文期刊名：Chemical Science

收录：EI(收录号：20132416423604)

语种：英文

外文关键词：Tissue - Mammals - Infrared devices - Probes - Quenching

摘要：We developed a near-infrared fluorescence probe for the highly selective and sensitive detection of NAT2 activity. An arylamine was designed as the substrate part for NATs, as well as a PET quencher for cyanine dye. CYP1 with a 4-position arylamine displayed efficient PET quenching, and exhibited high selectivity and sensitivity to NAT2 with no response to NAT1. For practical application, we demonstrated CYP1 could exhibit excellent performance for endogenous NAT2 fluorescence imaging in living mice. We further verified the NAT2 distribution in various tissues by detecting the response to the corresponding tissue homogenates. The results were consistent with the fluorescence imaging in vivo and previous research. Above all, the probe CYP1 could selectively and sensitively monitor the NAT2 activity in enzymatic system, living mice and tissue homogenate samples. Therefore, the results indicated that CYP1 has potential use for the detection of NAT2 activity in the clinic and in research. ? 2013 The Royal Society of Chemistry.