文献类型：期刊文献

中文题名：胰酶对皮肤角质细胞分离和传代的影响

英文题名：Effect of Trypsin on the Rat Keratinocyte Separation and Subculture

作者：欧阳安力[1];周燕[1];华平[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2002

卷号：18

期号：1

起止页码：59

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：MEDLINE(收录号：11977602);CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家高技术研究发展计划课题 (No .10 2 0 9 0 5 0 4);国家重点基础研究发展规划"组织工程的基本科学问题"项目的资助 (No .G19990 5 430 9)~~

语种：中文

中文关键词：胰酶;皮肤角质细胞;分离;传代;贴壁率;克隆形成率

外文关键词：keratinocyte, trypsin, attached ratio, colony forming efficiency

摘要：考察不同的胰酶浓度及其作用时间对角质细胞分离和传代的影响。实验发现在胰酶浓度 0 2 5 %时作用5min可获得的总活细胞量和有克隆形成能力的细胞量均优于其它培养条件 ;而在胰酶浓度 0 0 5 %时作用 5min可获得最大的原代角质细胞贴壁率。随着胰酶浓度的提高 ,传代角质细胞的贴壁率和贴壁速率常数以及克隆形成率均随之增加 ,因此在传代培养时使用 0 2 5
The effect of trypsin on the separation an subculture of the keratinocytes was investigated in this work.It was found that when 0 25% trypsin was employed for 5 minutes to separate keratinocytes,the number of active keratinocytes and the cells capable of forming colony were higher than those of other experimental conditions.The maximum attached ratio of primary keratinocytes was obtained when skin tissues were treated at 0 05% concentration of trypsin.With the increase of the trypsin concentrations,the attached ratio,attachment rate constant,and colony forming efficiency were all increased.Thus,0 25% concentration of trypsin was recommended for separating and subculturing the keratinocytes.