文献类型：期刊文献

英文题名：2-N, 6-O-sulfated chitosan-assisted BMP-2 immobilization of PCL scaffolds for enhanced osteoinduction

作者：Cao, Lingyan[1,2,3,4];Yu, Yuanman[1,2];Wang, Jing[1,2];Werkmeister, Jerome A.[3];McLean, Keith M.[3];Liu, Changsheng[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]CSIRO Mfg, Bayview Ave, Clayton, Vic 3168, Australia;[4]Shanghai Jiao Tong Univ, Dept Prosthodont, Coll Stomatol, Peoples Hosp 9,Sch Med, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China

年份：2017

卷号：74

起止页码：298

外文期刊名：MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20170203224560);WOS:【SCI-EXPANDED(收录号:WOS:000397356900035)】；

基金：The authors wish to express their gratitude for financial supports from the National Natural Science Foundation of China (No. 31330028, No. 31271011 and No. 31470923), Major Basic Research Foundation of Shanghai Science and Technique Committee (14JC1490800), and International Cooperation Project of Shanghai Science and Technique Committee (15520711100). This study was also supported by the 111 Project (B14018).

语种：英文

外文关键词：Electrospun; Sulfated chitosan; Bmp-2; Release; Bioactivity

摘要：The aim of this study was to develop a 2-N, 6-O-sulfated chitosan (26SCS) modified electrospun fibrous PCL scaffold for bone morphogenetic protein-2 (BMP-2) delivery to improve osteoinduction. The PCL scaffold was modified by an aminolysis reaction using ethylenediamine (ED) and 26SCS was immobilized via electrostatic interactions (PCL-N-S). Scaffolds were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements. In vitro BMP-2 adsorption and release kinetics indicated that modified PCL-N-S scaffolds showed higher levels of binding of BMP-2 (about 30-100 times), moderative burst release (about one third), and prolonged releasing time compared to the unmodified PCL scaffold. The bioactivity of released BMP-2 determined by alkaline phosphatase (ALP) activity assay was maintained and improved 8-12 times with increasing concentration of immobilized 26SCS on the scaffolds. In vitro studies demonstrated that bone marrow mesenchymal stem cells (BMSCs) attached more readily to the PCL-N-S scaffolds with increased spreading. In conclusion, 26SCS modified PCL scaffolds can be a potent system for the sustained and bioactive delivery of BMP-2. (C) 2017 Elsevier B.V. All rights reserved.