文献类型：期刊文献

英文题名：Dual-functional peptide conjugated gold nanorods for the detection and photothermal ablation of pathogenic bacteria

作者：Chen, Qingyu[1];Zhang, Liwei[1];Feng, Yonghai[1];Shi, Fan[2];Wang, Yibing[2];Wang, Ping[2];Liu, Lei[1]

机构：[1]Jiangsu Univ, Sch Mat Sci & Engn, Inst Adv Mat, Zhenjiang 212013, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg, Biomed Nanotechnol Ctr,Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2018

卷号：6

期号：46

起止页码：7643

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20184906202218);WOS:【SCI-EXPANDED(收录号:WOS:000451842200006)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21573097, 51503087 and 21606112), the China Postdoctoral Foundation Committee (No. 2016M600372), the Natural Science Foundation of Jiangsu Province (No. BK20160503), the Postdoctoral Fund of Jiangsu Province (No. 1601022A), the Natural Science Fund for Colleges and Universities in Jiangsu Province (No. 17KJB180001), and the Programs of Senior Talent Foundation of Jiangsu University (No. 15JDG137).

语种：英文

外文关键词：Ablation - Binding energy - Gold - Escherichia coli - Nanorods - Surface plasmon resonance - Biocompatibility

摘要：It is urgent to find an effective and convenient method to detect and kill microbial strains due to the threat of pathogenic bacterial infection and the low effectiveness of antibiotics. In the present work, a dual-functional peptide coupling gold nanoconjugate (Au@P937 NRs) was facilely fabricated by conjugating an adhesion peptide (WGLHTSATNLYLHGGGC, P937) with Au nanorods (Au NRs), of which the peptide P937 was designed by the phage display biopanning technology and showed specific binding affinity to bacteria. After the modification of P937, the longitudinal surface plasmon resonance (LSPR) absorbance peak of the Au@P937 NRs was found to be very sensitive to the variation of bacterial concentration. The Au@P937 NRs exhibited enhanced NIR-based photothermal conversion efficiency. Moreover, the Au@P937 NRs exhibited good biocompatibility. Hence, the Au@P937 NRs could be used as a dual-functional nanoconjugate for the detection and photothermal ablation of bacteria. For the bacterial detection by the Au@P937 NRs, the detection limit could reach 46 cfu mL(-1) for E. coli and 89 cfu mL(-1) for S. aureus, respectively. Both E. coli and S. aureus could be killed completely in 10 min by the Au@P937 NRs under 808 nm laser irradiation. This work provided a facile, convenient and effective strategy for detecting and sterilizing bacteria.