文献类型：期刊文献

英文题名：Inactivating NADH:quinone oxidoreductases affects the growth and metabolism of Klebsiella pneumoniae

作者：Zhang, Lijuan[1];Bao, Wenjing[1];Wei, Renquan[1];Fu, Shuilin[1];Gong, Heng[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：65

期号：6

起止页码：857

外文期刊名：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

收录：;EI(收录号：20183505759473);WOS:【SCI-EXPANDED(收录号:WOS:000454576300011)】；

基金：This work was supported by the National Natural Science Foundation of China under Grant No. 31271862.

语种：英文

外文关键词：1,3-propanediol; 2,3-butanediol; intracellular redox; Klebsiella pneumoniae; NADH:quinone oxidoreductase

摘要：NADH:quinone oxidoreductases (NQOs) act as the electron entry sites in bacterial respiration and oxidize intracellular NADH that is essential for the synthesis of numerous molecules. Klebsiella pneumoniae contains three NQOs (NDH-1, NDH-2, and NQR). The effects of inactivating these NQOs, separately and together, on cell metabolism were investigated under different culture conditions. Defective growth was evident in NDH-1-NDH-2 double and NDH-1-NDH-2-NQR triple deficient mutants, which was probably due to damage to the respiratory chain. The results also showed that K. pneumoniae can flexibly use NQOs to maintain normal growth in single NQO-deficient mutants. And more interestingly, under aerobic conditions, inactivating NDH-1 resulted in a high intracellular NADH:NAD(+) ratio, which was proven to be beneficial for 2,3-butanediol production. Compared with the parent strain, 2,3-butanediol production by the NDH-1-deficient mutant was increased by 46% and 62% in glycerol- and glucose-based media, respectively. Thus, our findings provide a practical strategy for metabolic engineering of respiratory chains to promote the biosynthesis of 2,3-butanediol in K. pneumoniae.