文献类型：期刊文献

英文题名：Targeted doxorubicin delivery to liver cancer cells by PEGylated mesoporous silica nanoparticles with a pH-dependent release profile

作者：Gu, Jinlou[1];Su, Shasha[1];Zhu, Mingjie[2];Li, Yongsheng[1];Zhao, Wenru[1];Duan, Yourong[2];Shi, Jianlin[1,3]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Canc Inst, Shanghai 200032, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China

年份：2012

卷号：161

起止页码：160

外文期刊名：MICROPOROUS AND MESOPOROUS MATERIALS

收录：;EI(收录号：20123015272323);WOS:【SCI-EXPANDED(收录号:WOS:000307085900021)】；

基金：This work was financially supported by the National Basic Research Program of China (973 Program, 2012CB933602), Research Fund for the Doctoral Program of Higher Education (No. 20090074120009), Natural Science Foundation of China (Nos. 51072053, 21001043) and the Fundamental Research Funds for the Central Universities (WD 1114004).

语种：英文

外文关键词：Targeting; Drug delivery; Pegylated; pH-dependent; Mesoporous silica nanoparticles

摘要：A layer of PEG (poly(ethylene glycol))-galactose was successfully grafted onto the external surface of mesoporous silica nanoparticles (MSNs) via a new silane-free approach, while the internal surface of MSNs was preserved for the encapsulation of the widely used anti-cancer drug of doxorubicin (DOX). The nanosized morphology and ordered structure of the synthesized drug delivery vehicles were verified by XRD and TEM observations. The successful grafting of PEG layer and peripherally exposed galactose ligands on the external surface of MSNs (abbreviated as MSNs-P/G) was confirmed by FT-IR and solid C-13 NMR. The high-density PEG layer effectively reduced the human serum protein (HSP) adsorbance to the surface of MSNs. The maximum DOX loading amount reached as high as 900 mg/g and the loaded drug released in a pH-dependent way. Both confocal laser scanning microscopy (CLSM) observation and flow cytometry measurements supported the facts that cellular uptake of MSNs-P/G was significantly higher than that of the pristine MSNs benefitting from the galactose-receptor-mediated endocytosis process. This was consistent with the higher cytotoxicity observed with the DOX@MSNs-P/G against the HepG2 cell line by MIT measurements. (C) 2012 Elsevier Inc. All rights reserved.