文献类型：期刊文献

中文题名：非光合固碳微生物菌群的耐盐特性及其影响因素

英文题名：Salt-tolerant characteristics of non-photosynthetic microbial community and its influencing factors

作者：宋红彪[1];胡佳俊[2];付小花[2];陆兵[1];徐殿胜[1];王磊[2]

机构：[1]华东理工大学生物工程学院,生物反应器工程国家重点实验室,上海200237;[2]同济大学环境科学与工程学院,污染控制与资源化研究国家重点实验室,上海200092

年份：2014

卷号：44

期号：6

起止页码：7

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：CSCD:【CSCD_E2013_2014】；

基金：国家863项目(编号:2012AA050101);国家自然科学基金项目(编号:21177093;21307093);高等学校博士学科点专项科研基金(编号:20130072110025);中国博士后科学基金(编号:2013M531220;2014T70430)

语种：中文

中文关键词：非光合固碳微生物菌群;盐碱地改良;耐盐特性;固碳效率

外文关键词：NPMC; improvement of alkali-saline land; salt-tolerance feature; CO2 fixation efficiency

摘要：从海洋中分离驯化得到的非光合固碳微生物菌群(NPMC)是无需光照和供氢的化能自养微生物,若能用于贫瘠盐碱地改良,实现其在盐碱土壤中的二次固碳,对于盐碱土壤的低碳化改良具有重要的意义。本实验初步验证了NPMC的耐盐特性,以及微量元素和磷酸盐缓冲液两单因素在次高盐条件下对NPMC固碳效率的影响。并通过响应面法研究了微量元素与盐浓度对NPMC固碳效率的交互作用。结果表明,非光合固碳微生物拥有耐受高盐浓度的特性,可耐受高达100g/L以上的总盐度,因此可用于重盐碱土壤的改良。微量元素和磷酸盐缓冲液浓度的增加,都可增强NPMC的固碳效率,微量元素的促进效应高于磷酸盐缓冲液。微量元素和盐度对NPMC固碳效率的影响存在交互作用。
The non-photosynthetic microbial community （NPMC） isolated from ocean are chemoautotroph microbes with- out providing photosynthetic and hydrogen. Using NPMC to improve alkali-saline land can achieve its secondary carbon fixation in saline soil and thus reach low-carbon improvement to saline-alkali soil. In this experiment, the salt-tolerance feature of NPMC was evaluated, and the effects of trace elements and phosphate buffer on the CO2 fixation efficiency of NPMC under sub-high-salt condition were also studied, respectively. The results showed that NPMC could tolerate high salt concentration up to 100 g/L, which was enough for the heavy saline-alkali land reclamation. Trace elements and phos- phate buffer could enhance the CO2 fixation efficiency of NPMC, respectively. Under the sub-high-salt condition, trace elements was more effective than phosphate buffer. In addition, there was interaction between trace elements and salinity for the CO2 fixation efficiency of NPMC.