文献类型：期刊文献

英文题名：Stimulation of trans-resveratrol biosynthesis in vitis vinifera cv. Kyoho cell suspension cultures by 2, 3-dihydroxypropyl jasmonate elicitation

作者：Shen, Xu[1]; Du, Qingshan[2]; Xu, Yufang[2]; Lu, Yanhua[1]

机构：[1] East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering, Shanghai, China; [2] East China University of Science and Technology, Institute of Pesticides and Pharmaceuticals, Shanghai Key Laboratory of Chemical Biology, Shanghai, China

年份：2012

卷号：15

期号：5

起止页码：3

外文期刊名：Electronic Journal of Biotechnology

收录：EI(收录号：20123915479553)

语种：英文

外文关键词：Resveratrol - Chemical activation - Metabolites - Amino acids - Plant cell culture - Cell signaling - Plants (botany) - Signal transduction

摘要：Background: Plant cell suspension culture of Vitis vinifera is a promising technology for investigating different factors that are able to induce and/or modify stilbenes biosynthesis. Jasmonates have been reported to play an important role in a signal transduction pathway that regulates defence responses as well as the production of secondary metabolites. In this study, 2, 3-dihydroxypropyl jasmonate (DHPJA) was used to investigate its effect on stimulating trans-resveratrol (t-R) accumulation and the plant defence responses in Vitis vinifera cv. Kyoho cell suspension cultures for the first time. Results: It demonstrated that DHPJA had superior effects on stilbenoids accumulation over methyl jasmonate (MeJA). The optimal condition was 150 μM DHPJA added on day 15 of cultivation period, with the highest level of t-R accumulation which was increased 1.8-fold and 1.3-fold compared with the control and 150 μM MeJA respectively. DHPJA induced stronger plant defence responses, including oxidative burst and activation of L-phenylalanine ammonia lyase (PAL) than MeJA. H2O2 generation induced by DHPJA played a significant role in enhancing t-R accumulation. Adding a specific inhibitor of H2O2 signalling pathway inhibited DHPJA-induced t-R accumulation, but had no effects on DHPJA-induced other metabolites accumulation, which resulted in regulations of product diversity. Conclusions: This study demonstrated that DHPJA was an efficient elicitor to enhance t-R accumulation by activating stronger oxidative burst, and H2O2 signalling pathway could regulate product diversity in DHPJA induced V. vinifera cv. Kyoho cell suspension cultures. ? 2012 by Pontificia Universidad Católica de Valparaíso, Chile.