文献类型：期刊文献

英文题名：Cost-efficient and process-efficient separation of geniposide from Gardenia jasminoides Ellis by high-performance counter-current chromatography

作者：Zhang, Min[1]; Ignatova, Svetlana[2]; Hu, Ping[3]; Liang, Qionglin[4]; Wang, Yiming[4]; Sutherland, Ian[2]; Jun, Frank Wu[5]; Luo, Guoan[1,4]

机构：[1] Modern Engineering Center for Traditional Chinese Medicine, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; [2] Brunel Institute for Bioengineering, Brunel University, Kingston Lane, Uxbridge, Middlesex UB8 3PH, United Kingdom; [3] School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; [4] Institute of Biomedicine, Tsinghua University, Beijing 100084, China; [5] XiangCam TCM Research Centre Ltd., Meditrina, Babraham Research Campus, Cambridge CB22 3AT, United Kingdom

年份：2012

卷号：89

起止页码：193

外文期刊名：Separation and Purification Technology

收录：EI(收录号：20121214886093)

语种：英文

外文关键词：Liquid chromatography - Medicine - Efficiency - Hardwoods - Cost effectiveness

摘要：Gardenia jasminoides Ellis has been used for traditional Chinese medicine to treat all forms of febrile diseases. A cost-efficient and process-efficient separation of the bioactive molecule geniposide from G. jasminoides Ellis by high-performance counter-current chromatography (HPCCC) has been developed. Separation by preparative HPCCC was performed with a two-phase solvent system composed of ethyl acetate-butanol-water (0.4:1.6:2, v/v). The separation yielded 445 mg of geniposide with a purity of 95.2% from 4.0 g of crude extract. A factor of cost efficiency was developed and used for an economic evaluation of the separation process. The cost efficiency for geniposide was 0.070 g h -1 $-1. The separation process by HPCCC was demonstrated to be more economical and efficient than those methods by HSCCC and LC. ? 2012 Elsevier B.V. All rights reserved.