文献类型：期刊文献

英文题名：Theoretical analysis of RNA polymerase fidelity: a steady-state copolymerization approach

作者：Fu, Wenbo[1];Li, Qiushi[1];Song, Yongshun[2];Shu, Yaogen[3];Ouyang, Zhongcan[4];Li, Ming[1]

机构：[1]Univ Chinese Acad Sci, Sch Phys Sci, Beijing 100049, Peoples R China;[2]East China Univ Sci & Technol, Sch Phys, Shanghai 200237, Peoples R China;[3]Univ Chinese Acad Sci, Wenzhou Inst, Wenzhou 325001, Peoples R China;[4]Chinese Acad Sci, Inst Theoret Phys, Beijing 100190, Peoples R China

年份：2022

卷号：74

期号：1

外文期刊名：COMMUNICATIONS IN THEORETICAL PHYSICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000728962500001)】；

基金：The authors thank the financial support by National Natural Science Foundation of China (No.11675180, 11774358), the CAS Strategic Priority Research Program (No.XDA17010504), Key Research Program of Frontier Sciences of CAS (No. Y7Y1472Y61), Research Fund of Wenzhou Institute CAS (No. WIUCASYJ2020004, WIUCASQD2020009).

语种：英文

外文关键词：RNA polymerase; fidelity; transcription; proofreading; steady-state copolymerization theory

摘要：The fidelity of DNA transcription catalyzed by RNA polymerase (RNAP) has long been an important issue in biology. Experiments have revealed that RNAP can incorporate matched nucleotides selectively and proofread the incorporated mismatched nucleotides. However, systematic theoretical researches on RNAP fidelity are still lacking. In the last decade, several theories on RNA transcription have been proposed, but they only handled highly simplified models without considering the high-order neighbor effects and the oligonucleotides cleavage both of which are critical for the overall fidelity. In this paper, we regard RNA transcription as a binary copolymerization process and calculate the transcription fidelity by the steady-state copolymerization theory recently proposed by us for DNA replication. With this theory, the more realistic models considering higher-order neighbor effects, oligonucleotides cleavage, multi-step incorporation and multi-step cleavage can be rigorously handled.