文献类型：期刊文献

英文题名：Single-step fabrication of catechol-Ε-poly-L-lysine antimicrobial paint that prevents superbug infection and promotes osteoconductivity of titanium implants

作者：Xu, Miao[1,2]; Song, Qing[1]; Gao, Lingling[1,2]; Liu, Huan[3]; Feng, Wei[1,2]; Huo, Jingjing[1]; Jin, Haoyu[1]; Huang, Lei[4]; Chai, Jin[1]; Pei, Yangyang[1]; Qu, Xue[3]; Li, Peng[1,2]; Huang, Wei[1,2]

机构：[1] Frontiers Science Center for Flexible Electronics [FSCFE], Shaanxi Institute of Flexible Electronics [SIFE], Shaanxi Institute of Biomedical Materials and Engineering [SIBME], Northwestern Polytechnical University [NPU], 127 West Youyi Road, Xi'an, 710072, China; [2] Key Laboratory of Flexible Electronics [KLOFE], Institute of Advanced Materials [IAM], Jiangsu National Synergetic Innovation Center for Advanced Materials [SICAM], Nanjing Tech University [NanjingTech], Nanjing, 211816, China; [3] Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 200237, China; [4] The State Key Laboratroy of Functions & Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China

年份：2020

卷号：396

外文期刊名：Chemical Engineering Journal

收录：EI(收录号：20202108692010)

语种：英文

外文关键词：Molluscs - Metal implants - Amino acids - Biomimetics - Tissue - Hydroxyapatite - Paint - Phenols - Body fluids - Titanium

摘要：Prevention of infection and promotion of osteoconduction are the key factors to achieve long-term success in orthopaedic implants. In this work, an effective antimicrobial and osteoconductive coating is facilely prepared by the cross-linking of Ε-poly-L-lysine (EPL) and catechol (abbreviated as EPLC) via a one-step mussel-inspired chemistry. A series of EPLC paints were prepared by catechol (15 mM) and varying concentrations of EPL (30, 40, and 50 mM) and named as EPLC1, EPLC2, and EPLC3. The coatings were easy to paint on implants. EPLC3 with the highest EPL concentration exhibited the most potent antimicrobial activity (greater than 99.99%) against both Gram-negative bacteria Escherichia coli (E. coli) and Gram-positive "superbug" methicillin-resistant Staphylococcus aureus (MRSA), and inhibited the biofilm formation of MRSA up to one week. Furthermore, the EPLC3 painted Ti implant was capable of reducing more than 3-log bacterial burden in a rodent subcutaneous infection model infected by MRSA. More interestingly, EPLC3 coating increased the osteoconductivity of Ti surfaces by promoting mouse pre-osteoblast MC3T3-E1 adhesion and proliferation. And the EPLC3 painted Ti surfaces could induce hydroxyapatite (HAp) formation after incubation in simulating human body fluids (SBF) within two weeks. Overall, the mussel-inspired EPLC paint can be easily applied to orthopaedic implants, and endowed Ti with antimicrobial activity and promoted its osteoconductivity, which will benefit the long-term success of Ti implants. ? 2020 Elsevier B.V.