文献类型：期刊文献

英文题名：An effective established biosensor of bifunctional probes-labeled AuNPs combined with LAMP for detection of fish pathogen Streptococcus iniae

作者：Zhou, Ya[1,2];Xiao, Jingfan[1,2];Ma, Xin[1,2];Wang, Qiyao[1,2,3];Zhang, Yuanxing[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2018

卷号：102

期号：12

起止页码：5299

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20181805119843);WOS:【SCI-EXPANDED(收录号:WOS:000432692300025)】；

基金：This work was supported by grants from the Ministry of Agriculture of China (nos. CARS-47-G17 and nyhyzx-201303047), the National Natural Sciences Foundation of China (no. 31672696), and the Project of Marine Public Welfare Profession (no. 201405003-2).

语种：英文

外文关键词：LAMP; Gold nanoparticles; Bifunctional probes; Streptococcus iniae

摘要：In purpose of valid Streptococcus iniae detection, we established a colorimetric biosensor using gold nanoparticles (AuNPs) labeled with dual functional probes and along with loop-mediated isothermal amplification (LAMP) assay (LAMP-AuNPs). Based on the characteristics of self-aggregation and bio-conjugation with ligands, AuNPs were chosen for observable color change in tandem with LAMP amplification method to reach high sensitivity and easy operation. Meanwhile, the improvement of dual probes that could fully utilize the LAMP product gave the biosensor a stable result exhibition. LAMP-AuNPs targeting gene ftsB, one of the ATP transporter-related genes, turned out favorable specificity in cross reaction among other fish pathogens. The detect limit of 10(2) CFU revealed a better sensitivity compared with polymerase chain reaction (PCR) method and AuNPs lateral flow test strip (LFTS). It was also proved to be effective by zebrafish infection model trials with less than 2-h time consumption and nearly no devices which make it a convenient biosensor for point-to-care S. iniae detection.