文献类型：期刊文献

中文题名：耐碳源分解代谢产物调节的螺旋霉素产生菌的理性化筛选

英文题名：RATIONAL SCREENING OF THE SPIRAMYCIN-PRODUCING STRAIN STREPTOMYCES SPIRAMYCETICUS RESISTING TO CARBON CATABOLITE REPRE-SSIONIRG

作者：罗镜青[1];李友荣[2]

机构：[1]武汉大学生物系;[2]华东化工学院生化工程系

年份：1989

卷号：14

期号：4

起止页码：243

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：理性化筛选;螺旋霉素;紫外线诱变

外文关键词：Rational screening; 2-deoxy-D-glucose; Spriamycin; U.V. mutation

摘要：通过紫外线处理和在2-脱氧-D-葡萄糖存在下,筛选耐葡萄糖分解代谢物调节的突变株。本实验得到一株生产能力明显提高的菌株,在一定的2-脱氧-D-葡萄糖浓度下,生产能力可提高78.9％。
Spiramycin produced by Streptomyces spiramyceticus A. sp. 799 is Subject to glucose catabolite repression. After the suspension of spores was treated by UV, we isolated the resistant strains which could grow on the medium contain ing 2 -deoxy-D-glucose. The mutaats obtained by above method produced much more spiram-ycin than those obtained by random screening after UV treatment. The productivity of mutants can be increased 78. 9 % in a certain concentration of 2 -deoxy-D-gluco-se.