文献类型：期刊文献

英文题名：Engineered Extracellular Vesicle-Encapsuled Nanoreactors for Effective Targeting and Cascade Killing of Cancer Cells

作者：Wu, Man-Sha[1];Zhou, Ze-Rui[1];Wang, Xiao-Yuan[1];Lv, Jian[1];Li, Da-Wei[1];Qian, Ruo-Can[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat,Joint Int Lab, Shanghai 200237, Peoples R China

年份：2023

卷号：6

期号：4

起止页码：1479

外文期刊名：ACS APPLIED BIO MATERIALS

收录：EI(收录号：20231413832152);WOS:【ESCI(收录号:WOS:000971215100001)】；

基金：ACKNOWLEDGMENTS This research was supported by the National Natural Science Foundation of China (21977031) , Science and Technology Commission of Shanghai Municipality (2018SHZDZX03) , Shanghai Science and Technology Committee (22ZR1416800, 19ZR1472300) , and the Fundamental Research Funds for the Central Universities. The authors thank the Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：extracellular vesicles; polydopamine; biomarkers; nanomaterials; cancer

摘要：Nanomaterials have presented great potential for cancer therapy. However, their therapeutic efficacy is not always satisfied because of inefficient biocompatibility and targeting efficacy. Here, we report engineered extracellular vesicle (EV)-encapsuled nanoreactors for the targeting and killing of cancer cells. EVs are extracted from engineered cancer cells with surface N-glycans cut and intracellular microRNA-21 (miR-21) silenced to generate cancer-targeting membranes for the following coating of gold-polydopamine (PDA) core-shell nanoparticles. The encapsuled nanoparticles are decorated with doxorubicin (Dox), glucose oxidase (GOx), and miR-21-indicative DNA tags. Once endocytosed, the acidic pH, together with the photothermal effect of the PDA shell, can promote the release of Dox and GOx-catalyzed H2O2 generation/glucose consumption, while the DNA tags allow enhanced fluorescence imaging of miR-21 to indicate the targeting effect. The coadministration of EV-assisted delivery and cascade treatment represents a promising strategy for combination therapy.