文献类型：期刊文献

英文题名：Synthesis and evaluation of a maltose-bonded silica gel stationary phase for hydrophilic interaction chromatography and its application in Ginkgo Biloba extract separation in two-dimensional systems

作者：Sheng, Qianying[1];Yang, Kaiya[1];Ke, Yanxiong[2];Liang, Xinmiao[2];Lan, Minbo[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Pharmaceut Proc Chem, Engn, Shanghai, Peoples R China

年份：2016

卷号：39

期号：17

起止页码：3339

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20163702798109);WOS:【SCI-EXPANDED(收录号:WOS:000383701000007)】；

基金：This work was supported by the Natural Science Foundation of Shanghai (16ZR1407600), Shanghai Sailing Program (16YF1402400), the Fundamental Research Funds for the Central Universities (222201514022), and the National Natural Science Foundation of China (21304034).

语种：英文

外文关键词：Ginkgo Biloba extract; Hydrophilic interaction liquid chromatography; Saccharide-modified stationary phases; Separation selectivity; Two-dimensional chromatography

摘要：Maltose covalently bonded to silica was prepared by using carbonyl diimidazole as a crosslinker and employed as a stationary phase for hydrophilic interaction liquid chromatography. The column efficiency and the effect of water content, buffer concentration, and pH value influenced on retention were investigated. The separation or enrichment selectivity was also studied with nucleosides, saccharides, amino acids, peptides, and glycopeptides. The results indicated that the stationary phase processed good separation efficiency and separation selectivity in hydrophilic interaction liquid chromatography mode. Moreover, a two-dimensional hydrophilic interaction liquid chromatography x reversed-phase liquid chromatography method with high orthogonality was developed to analyze the Ginkgo Biloba extract fractions. The development of this two-dimensional chromatographic system would be an effective tool for the separation of complex samples of different polarities and contents.