文献类型：期刊文献

英文题名：Quantitative evaluation of the shear threshold on Carthamus tinctorius L. cell growth with computational fluid dynamics in shaken flask bioreactors

作者：Liu, Yu[1,2];Wang, Ze-Jian[1,2,3];Zhang, JianWen[1,2];Xia, Jian-ye[1,2];Chu, Ju[1,2];Zhang, Si-Liang[1,2];Zhuang, Ying-Ping[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Inst Biomfg Technol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Collaborat Innovat Ctr, Shanghai 200237, Peoples R China;[3]Delft Univ Technol, Dept Biotechnol, NL-2628 BC Delft, Netherlands

年份：2016

卷号：113

起止页码：66

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20162502504264);WOS:【SCI-EXPANDED(收录号:WOS:000380869600009)】；

基金：This work was financially supported by the National High Technology Research and Development Program (2015AA021005), and 973 Program No. 2013CB733600. the Royal DSM and partially supported by NOW-MoST Joint Program (2013DFG32630). We also thank pharmacy corporation Practical Bio Co., Ltd. (DALIAN, China) for donating the industrial strain.

语种：英文

外文关键词：Fluid mechanics; Power consumption; Aggregation; Plant cell bioreactors; C. tinctorius L. cell; Stress thresholds

摘要：Quantitative evaluation of the shear threshold on C. tinctorius L. cell growth is vital for bioreactor system design and optimization of scaled-up industrial cultivation. The present research focused on investigating the effects of shear force on C. tinctorius L cell growth by computational fluid dynamic (CFD) analysis in shaken flasks. The results revealed that specific cell growth rates were greatly inhibited as the shear force increased from 1.17 to 2.42 Pa. Recovery of viability and aggregation diameter to their normal levels could be implemented after a 4-day adaptation with large fluctuations in physiological state. With further correlation analysis on shear force and C. tinctorius L. growth rate, a threshold value was identified at an average and maximum shear stress of approximately 0.55 Pa (0.06 w/kg) and 4.00 Pa (0.93 w/kg) according to the influence on cell growth. Quantitative data on shear effects can facilitate the design of industrial processes and lead to more rational scale-up in industrial C. tinctorius L cultivation. (C) 2016 Elsevier B.V. All rights reserved.