文献类型：期刊文献

英文题名：Nanopipette-Based Nanosensor for Label-Free Electrochemical Monitoring of Cell Membrane Rupture under H2O2 Treatment

作者：Wang, Xiao-Yuan[1,2];Lv, Jian[1,2];Hong, Qin[1,2];Zhou, Ze-Rui[1,2];Li, Da-Wei[1,2];Qian, Ruo-Can[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2021

卷号：93

期号：41

起止页码：13967

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20214311058441);WOS:【SCI-EXPANDED(收录号:WOS:000710444000028)】；

基金：This research was supported by the National Natural Science Foundation of China (21977031, 21777041, and 21974046), the National Science and Technology Major Project of China (2018ZX10302205), the Shanghai Science and Technology Committee (19ZR1472300), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Cell death - Cell membranes - Cell signaling - Nanosensors

摘要：H2O2 is an essential signaling molecule in living cells that can cause direct damage to lipids, proteins, and DNA, resulting in cell membrane rupture. However, current studies mostly focus on probe-based sensing of intracellular H2O2, and these methods usually require sophisticated probe synthesis and instruments. In particular, local H2O2 treatment induces cell membrane rupture, but the level of cell membrane destruction is unknown because the mechanical properties of the cell membrane are difficult to accurately determine. Therefore, highly sensitive and label-free methods are required to measure and reflect mechanical changes in the cell membrane. Here, using an ultrasmall quartz nanopipette with a tip diameter less than 90 nm as a nanosensor, label-free and noninvasive electrochemical single-cell measurement is achieved for real-time monitoring of cell membrane rupture under H2O2 treatment. By spatially controlling the nanopipette tip to precisely approach a specific location on the membrane of a single living cell, stable cyclic membrane oscillations are observed under a constant direct current voltage. Specifically, upon nanopipette advancement, the mechanical status of the cell membrane can be sensibly displayed by continuous current versus time traces. The electrical signals are collected and processed, ultimately revealing the mechanical properties of the cell membrane and the degree of cell apoptosis. This nanopipette-based nanosensor paves the way for developing a facile, label-free, and noninvasive strategy to assay the mechanical properties of the cell membrane during external stimulation at the single-cell level.