文献类型：期刊文献

英文题名：Gene cloning and characterization of ferritin H and M subunits from large yellow croaker (Pseudosciaena crocea)

作者：Zhang, Xiao[1];Wei, Wei[1];Wu, Haizhen[1];Xu, Hongyan[1];Chang, Kangmei[2];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Ocean Univ, Coll Ocean Sci, Zhoushan 316004, Zhejiang, Peoples R China

年份：2010

卷号：28

期号：5-6

起止页码：735

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000277822100001)】；

基金：This work was supported by the National High Technology Research and Development Program of China (2006AA09Z436), Ministry of Agriculture of China (Nos. nyhyzx07-046 and nycytx-50-G08), Chinese Postdoctoral Science Foundation (20060400172), and Shanghai Science and Technology Development Funds (No. 08QA14024) and Shanghai Leading Academic Discipline Project (No. B505).

语种：英文

外文关键词：Ferritin; Immune-associated; Large yellow croaker; Attenuated live Vibrio anguillarum

摘要：Ferritin, an iron-binding protein, plays a role in host immune response to invading stimulus. Two ferritin subunit cDNAs, lycfh and lycfm, were identified from large yellow croaker, Pseudosciaena crocea. Analysis of the lycfh and lycfm 5'-UTR showed that both had a putative iron responsive element (IRE). Amino acid sequence alignment and phylogenetic analysis indicated that LycfH and LycfM had high identity to H and M type subunit of vertebrate ferritins respectively, while both of them showed the low identity to L type subunit. In all eight tissues examined, lycfh and lycfm expressions were detectable on transcriptional level. Real-time PCR assay indicated that the transcriptional levels of lycfh and lycfm were significantly increased in liver, spleen and kidney of the fish stimulated by attenuated live Vibrio anguillarum strain. Furthermore, ferritin subunit H and M were probably two candidates of immune molecules involved in acute phase response in large yellow croaker because their transcriptional levels were quickly increased and reached the peak levels at 12 h in liver after challenged with bacteria. (C) 2009 Elsevier Ltd. All rights reserved.