文献类型：期刊文献

英文题名：The photoswitchable ABAMIs for the future regulation of GWT1 in a spatiotemporal level

作者：Ding, Qian[1];Li, Zongying[2];Xiao, Zaiwei[1];Zhang, Siting[1];Liu, Zimai[1];Shao, Xiaoguang[3];Li, Zhong[1,4];Fu, Wen[1,5];Shao, Xusheng[1,4,6,7]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai, Peoples R China;[2]Shandong Univ, Sch Pharmaceut Sci, Jinan, Shandong, Peoples R China;[3]Agr Comprehens Serv Ctr Xiaqiu Town, Yantai, Shandong, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[5]Guizhou Univ, State Key Lab Green Pesticide, Guiyang, Guizhou, Peoples R China;[6]East China Univ Sci & Technol, Sch Pharm, Shanghai Frontier Sci Res Base Optogenet Tech Cell, Shanghai, Peoples R China;[7]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai, Peoples R China

年份：2026

外文期刊名：SMART MOLECULES

收录：WOS:【ESCI(收录号:WOS:001757952500001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (32402439, 32472610), Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission), the project of State Key Laboratory of Green Pesticide (SKL-GPL-KF-202405), Instrument Improvement Funds of Shandong University (ts20250204), and Shandong Provincial Natural Science Foundation (ZR2025QC1309).

语种：英文

外文关键词：azobenzene-aminopyridine; bioactivity; GWT1; pesticide discovery; photoswitchable

摘要：Photoswitchable bioactive molecules have attracted more attention and are widely used as photochromic ligands (PCLs) in both agrochemicals and pharmaceuticals. The glycosylphosphatidylinositol (GPI) acyltransferase GWT1 has been identified as a novel agricultural target of the 2-aminopyridine fungicides. Herein, the GWT1-targeted azobenzene-aminopyridine derivatives (ABAMIs) were designed and synthesized to spatiotemporally regulate biological functions, which are promising tools in revealing the unclear antifungal mechanism. The maximal nine-fold difference in activity was found between UV-irradiated and unirradiated ABAMI1 against Botrytis cinerea (B. cinerea). This, in principle of concept, could realize the regulation of GWT1 functions. Furthermore, a three-fold bioactivity difference against Sclerotinia sclerotiorum (S. sclerotiorum) was observed between the trans- and cis-p F 4 ABAMIs that were sensitive to visible light. The ABAMIs were successfully developed as PCLs that were allowed to modulate fungicidal activity by light, providing innovative and powerful tools for understanding functional GWT1 and opening an avenue to the GWT1-targeted pesticide discovery.