文献类型：期刊文献

英文题名：The Molecular Ecology Analysis of Microbial Communities in Waste Water-based Mud

作者：Yang, Z. X.[1];Zhou, Y. B.[2];Xiang, X. Z.[3];Zhu, Z. B.[3];Pen, L.[3];Luo, Y. W.[3];Lu, J.[1,2]

机构：[1]E China Univ Sci & Technol, Shanghai 200237, Peoples R China;[2]Minist Educ, Key Lab Coal Gasificat & Energy Chem Engn, Shanghai, Peoples R China;[3]China Oilfield Serv Ltd, Beijing, Peoples R China

年份：2013

卷号：31

期号：9

起止页码：887

外文期刊名：PETROLEUM SCIENCE AND TECHNOLOGY

收录：;EI(收录号：20131716227014);WOS:【SCI-EXPANDED(收录号:WOS:000317292600001)】；

基金：The authors are grateful for the financial support provided by the National Natural Science Foundation of China (No. 51208201), China Postdoctoral Science Foundation (No. 2011M500744) and the Fundamental Research Funds for the Central Universities (No. WB1114033).

语种：英文

外文关键词：16S rRNA; diversity of microbial communities; PCR; phylogenetic analysis; waste waterbased mud

摘要：The molecular ecology analysis of microorganisms is important for the development of biological treatment of waste waterbased mud. The bacterial 16S rRNA gene clone library was constructed to detect the community diversity in waste drilling fluid in this study. The phylogenetic analysis indicated that most of clones were clustered in , -proteobacteria with the phylotypes belonging to -proteobacteria accounting for 78.6% of the clone library. Halomonas sp. and Chromohalobacter sp. as moderately halophilic bacteria were the main bacteria in waste waterbased mud, accounting for 47.8% of the total of clones. This study provides the certainty of chance of the improvement of biological treatment by the selection of dominant strain.