文献类型：期刊文献

英文题名：Rapid and high-throughput purification of salvianolic acid B from Salvia miltiorrhiza Bunge by high-performance counter-current chromatography

作者：Zhang, Min[1,2]; Ignatova, Svetlana[3]; Liang, Qionglin[2]; Wu Jun, Frank[4]; Sutherland, Ian[3]; Wang, Yiming[2]; Luo, Guoan[1,2]

机构：[1] Modern Engineering Center for Traditional Chinese Medicine, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China; [2] Institute of Biomedicine, Tsinghua University, Beijing, 100084, China; [3] Brunel Institute for Bioengineering, Brunel University, Kingston Lane, Uxbridge, Middlesex UB8 3PH, United Kingdom; [4] XiangCam TCM Research Centre Ltd., Unit 16, Meditrina, Babraham Research Campus, Cambridge, CB22 3AT, United Kingdom

年份：2009

卷号：1216

期号：18

起止页码：3869

外文期刊名：Journal of Chromatography A

收录：EI(收录号：20091512020736)

语种：英文

外文关键词：Throughput - Organic solvents - Efficiency - Liquid chromatography

摘要：A large-scale purification of salvianolic acid B from Salvia miltiorrhiza Bunge is presented. The method development began with selection of the solvent system, then optimization of the operating parameters and ended up with linear scale-up from an analytical to a preparative instrument. Three factors were used for method optimization and scale-up estimation: purity, process throughput and process efficiency. Preparation was achieved using a two-phase solvent system comprising hexane-ethyl acetate-methanol-acetic acid-water (1:5:1.5:0.00596:5, v/v). This preparation yielded 475 mg of salvianolic acid B with a purity of 96.1% from 1.5 g of crude extract. The process throughput of crude was 2.23 g/h while process efficiency per gram of target compound was 0.769 g/h. Two factors-process environmental risk factor and process evaluation factor were used for evaluation of the separation process. ? 2009 Elsevier B.V. All rights reserved.