文献类型：期刊文献

英文题名：Unusually Broad Substrate Profile of Self-Sufficient Cytochrome P450 Monooxygenase CYP116B4 from Labrenzia aggregata

作者：Yin, Yue-Cai[1];Yu, Hui-Lei[1,2];Luan, Zheng-Jiao[1];Li, Ren-Jie[1];Ouyang, Peng-Fei[1];Liu, Jing[1];Xu, Jian-He[1,2]

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Synthet Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2014

卷号：15

期号：16

起止页码：2443

外文期刊名：CHEMBIOCHEM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000344357600018)】；

基金：This work was financially supported by the National Natural Science Foundation of China (no. 20902023) and the Ministry of Science and Technology, China (no. 2011CB710800).

语种：英文

外文关键词：cytochromes; data mining; hydroxylation; oxidoreductases; protein engineering; substrate promiscuity

摘要：A new member of the CYP116B subfamilyP450(LaMO)was discovered in Labrenzia aggregata by genomic data mining. It was successfully overexpressed in Escherichia coli, purified, and subsequently characterized spectroscopically, and its catalytic properties were assessed. Substrate profiling of the P450(LaMO) revealed that it was a versatile catalyst, exhibiting hydroxylation and epoxidation activities as well as O-dealkylation and asymmetric sulfoxidation activities. Diverse compounds, including alkylbenzenes, aromatic bicyclic molecules, and terpenoids, were shown to be hydroxylated by P450(LaMO). Such diverse catalytic activities are uncommon for the bacterial P450s, and the P450(LaMO) -mediated stereoselective hydroxylation of inactivated CH bondsubiquitous and relatively unreactive in organic moleculesis particularly unusual. The self-sufficient nature of P450(LaMO), coupled with its broad substrate range, highlights it as an ideal template for directed evolution towards various applications.