文献类型：期刊文献

英文题名：Adenosine detection by using gold nanoparticles and designed aptamer sequences

作者：Li, Fan[2];Zhang, Juan[1];Cao, Xuni[2];Wang, Lihua[1];Li, Di[1];Song, Shiping[1];Ye, Bangce[2];Fan, Chunhai[1]

机构：[1]Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：134

期号：7

起止页码：1355

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000267499800014)】；

基金：This work was financially supported by National Natural Science Foundation ( 20704043, 20776039), the Program for New Century Excellent Talents (NCET-07-0287), Shanghai Natural Science Foundation (07ZR14136), Shanghai Shuguang Program (06SG32).

语种：英文

摘要：Based on gold nanoparticles (AuNPs) and engineered DNA aptamers, we designed a novel bioassay strategy for the detection of adenosine as a small target molecule. In this design, an aptamer is engineered to consist of two pieces of random-coil like ssDNA which are respectively attached to AuNPs through their 50-thiol-modified end. They can reassemble into the intact aptamer tertiary structure and induce nanoparticle aggregation in the presence of the specific target. Results have demonstrated that gold nanoparticles can effectively differentiate these two different DNA structures via their characteristic surface plasmon resonance-based color change. With this method, adenosine can be selectively detected in the low micromolar range, which means that the strategy reported here can be applicable to the detection of several other small target molecules.