文献类型：期刊文献

中文题名：谷氨酰胺和丙酮酸钠对NK-92细胞体外扩增的影响

英文题名：Promotion of NK-92 cells expansion by glutamine and sodium pyruvate

作者：应丹妮[1];陈焕芸[1];付岩[1];蔡海波[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2020

卷号：34

期号：3

起止页码：728

中文期刊名：高校化学工程学报

外文期刊名：Journal of Chemical Engineering of Chinese Universities

收录：CSTPCD;;EI(收录号：20203109004286);Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

语种：中文

中文关键词：丙酮酸钠;谷氨酰胺;自然杀伤细胞;体外扩增

外文关键词：sodium pyruvate;glutamine;natural killer-92 cells;expansion in vitro

摘要：为了优化免疫细胞体外培养体系实现其高效扩增,以自然杀伤细胞系(natural killer-92 cells,NK-92)为对象,通过氨基酸代谢分析及正交实验设计优化了关键代谢物浓度,并在有/无血清培养基中进行了验证。结果表明,谷氨酰胺丙酮酸钠浓度对NK-92细胞的体外扩增有显著影响,其最适浓度为13.0和2.5 mmol·L^-1。在此浓度下,有血清培养体系中NK-92细胞扩增15 d后,其扩增倍数可达到(3712.85±225.74)倍,显著高于优化前对照组的(2255.93±243.00)倍(p<0.05);其杀伤活性均值由57.23%提高到63.53%;无血清体系中NK-92细胞扩增15 d后,其扩增倍数可达到(3193.59±199.99)倍,明显高于对照组的(1917.16±242.87)倍(p<0.05),且显著提高了扩增后NK-92细胞中CD3-CD56+细胞比例和杀伤活性(p<0.05)。该结果可为免疫效应细胞无血清培养基的开发提供技术支持。
In order to achieve efficient expansion of immune cells in vitro,the concentration of key metabolites were optimized based on amino acid metabolism analysis of the natural killer-92 cells(NK-92)and orthogonal design.The results are validated in serum and serum-free medium,which indicate that glutamine and sodium pyruvate have significant effects on NK-92 expansion and the optimal concentrations are 13.0 and 2.5 mmol·L^-1 respectively.After culturing for 15 days under the concentrations,the total cell expansion of NK-92 cells in serum medium was(3712.85±225.74)folds,which was significantly higher than(2255.93±243.00)folds in the control group(p<0.05).The average cytotoxicity increased from 57.23%to63.53%.The total cell expansion of NK-92 cells in serum-free medium was(3193.59±199.99)folds,which was significantly higher than(1917.16±242.87)folds in the control group after culturing for 15 days(p<0.05).The proportion of CD3-CD56+cells and average cytotoxicity were also higher than that of the control group(p<0.05).These results provide technical supports for the development of serum-free media for immune effector cells.