文献类型：期刊文献

中文题名：抗逆性木霉菌株的快速筛选方法

英文题名：A rapid method for screening Trichodema strains with stress tolerance

作者：王强强[1];窦恺[1];陈捷[1];杨合同[2];王伟[3];章初龙[4]

机构：[1]上海交通大学农业与生物学院国家微生代谢重点实验室农业部都市农业(南方)重点实验室,上海200240;[2]山东省科学院生态研究所,济南250014;[3]华东理工大学生物工程研究所生物反应器工程国家重点实验室,上海200237;[4]浙江大学农业与生物技术学院,杭州310058

年份：2018

卷号：36

期号：4

起止页码：41

中文期刊名：上海交通大学学报（农业科学版）

外文期刊名：Journal of Shanghai Jiaotong University(Agricultural Science)

收录：CSTPCD

基金：国家重点研发计划(2017YFD0201108);国家科技基础性工作专项(2014FY120900)

语种：中文

中文关键词：木霉菌;耐受性;快速筛选

外文关键词：Trichoderma;tolerance;rapid screening

摘要：为了高效筛选耐受高温胁迫(36℃),低温胁迫(14℃),盐胁迫(1mol/L NaCl)的木霉菌株,采用12孔板结合菌落面积自动扫描仪,对8种(24株)木霉菌在上述逆境因子下的反应差异进行测定,构建逆境胁迫下优良菌株快速筛选和评价方法。通过相关分析表明,12孔板中菌落直径与相应菌株在9cm平板上的菌落直径呈正相关(P<0.05);通过线性回归分析得到,平板上菌落直径(Y)和12孔板中菌落直径(X)的线性回归方程分别为:Y=3.353 X+0.564,(36℃);Y=3.980 X-0.166,(14℃);Y=3.537 X+0.422,(1mol/L NaCl)。与传统筛选方法相比,快速筛选方法可以有效提高筛选效率并减少耗材。本研究关于木霉菌多孔板高效筛选抗逆菌株的技术对于今后科学利用木霉菌资源提供了重要依据和方法。
For efficient screening of Trichoderma with tolerance to the various environment stress factors including extreme temperatures（36 and 14 ℃）,salinity（1 mol/L NaCl）etc,a rapid method is extensively required.A total of 24 Trichodermaisolates from different species under different stresses were examined by using 12-well plate combined with the computer-based automatic microscopic scanning of colony area.The results showed that the colony diameters on 12-well plates（X）were positively correlated（P0.05）with those of the corresponding strains on 9 cm plates（Y）and the linear regression equations were Y=3.353 X＋0.564,Y=3.980 X＋0.166,Y=3.537 X＋0.422 under 36 ℃,14 ℃ and 1 mol/L NaCl stresses,respectively.Compared with the traditional screening methods,the new strategy significantly improved the time efficiency and consumed much less medium.In conclusion,the establishment of Trichoderma rapid screening approach provided the essential basis for future scientific evaluation and utilization of Trichoderma.