文献类型：期刊文献

英文题名：A new fermentation strategy for S-adenosylmethionine production in recombinant Pichia pastoris

作者：Zhang, Jianguo[1]; Wang, Xuedong[1]; Su, Erzheng[1]; Fang, Guochen[1]; Ren, Yuhong[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and Technology, No. 130 Meilong Road, Shanghai, 200237, China

年份：2008

卷号：41

期号：1

起止页码：74

外文期刊名：Biochemical Engineering Journal

收录：EI(收录号：20082611335861)

语种：英文

外文关键词：Cytology - Yeast - Fermentation - Bottles - Cells

摘要：A recombinant Pichia pastoris MutS expressing SAM2 gene of Saccharomyces cerevisiae was cultured for S-adenosylmethionine (SAM) accumulation. Effect of the amount of methanol added (0.5%, 1.0%, 2.0%, 3.0%, 4.0%, 6.0%, 10.0%, and 12.0%) and cell densities (9.57, 13.47, 21.74, 30.90, and 41.24 g/L dry cell weight (DCW)) on yield of SAM was found in flask cultivations. In flask experiments, maximal yield of SAM (1.29 g/L) was obtained at 2.0% methanol added and 30.90 g/L DCW which gave the maximal methanol consumption rate. Conjunct effect of amount of methanol added and cell density was found through Origin 7.0 (7.0 Microcal, USA). Scale up in 3.7 L bioreactor, 51% specific yield of SAM was enhanced at 0.6% methanol compared to that of 0.1% methanol. In fed-batches of different cell densities at 0.6% methanol, maximal yield of SAM was 8.66 g/L at 100 g/L DCW with 64% yield of SAM enhanced again. Methanol consumption rate at 100 g/L DCW was 4.81 mL/L h. Maintenance coefficient of 100 g/L DCW was lower than that of others significantly, although methanol consumption rate of 90 g/L DCW was higher (5.07 mL/L h) than that of 100 g/L DCW. ? 2008.