文献类型：期刊文献

英文题名：In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors

作者：Lim, Shey-Li[1];Voon, Chia Pao[1];Guan, Xiaoqian[1];Yang, Yi[2];Gardestrom, Per[3];Lim, Boon Leong[1,4,5]

机构：[1]Univ Hong Kong, Sch Biol Sci, Pokfulam, Hong Kong, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, Synthet Biol & Biotechnol Lab, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[3]Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, SE-90187 Umea, Sweden;[4]Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China;[5]HKU Shenzhen Inst Res & Innovat, Shenzhen, Peoples R China

年份：2020

卷号：11

期号：1

外文期刊名：NATURE COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000544946500003)】；

基金：This project was supported by the National Natural Science Foundation of China (31870212), the Seed Funding Program for Basic Research (201811159131), the Hong Kong Research Grants Council Area of Excellence Scheme (AoE/M-403/16), and the Innovation and Technology Fund (Funding Support to State Key Laboratory of Agrobiotechnology) of the HKSAR, China. Any opinions, findings, conclusions, or recommendations expressed in this publication do not reflect the views of the Government of the Hong Kong Special Administrative Region or the Innovation and Technology Commission."

语种：英文

摘要：The challenge of monitoring in planta dynamic changes of NADP(H) and NAD(H) redox states at the subcellular level is considered a major obstacle in plant bioenergetics studies. Here, we introduced two circularly permuted yellow fluorescent protein sensors, iNAP and SoNar, into Arabidopsis thaliana to monitor the dynamic changes in NADPH and the NADH/NAD(+) ratio. In the light, photosynthesis and photorespiration are linked to the redox states of NAD(P)H and NAD(P) pools in several subcellular compartments connected by the malate-OAA shuttles. We show that the photosynthetic increases in stromal NADPH and NADH/NAD(+) ratio, but not ATP, disappear when glycine decarboxylation is inhibited. These observations highlight the complex interplay between chloroplasts and mitochondria during photosynthesis and support the suggestions that, under normal conditions, photorespiration supplies a large amount of NADH to mitochondria, exceeding its NADH-dissipating capacity, and the surplus NADH is exported from the mitochondria to the cytosol through the malate-OAA shuttle. NADP(H) and NAD(H) are crucial energy molecules in plant metabolism. Here, via the use of circularly permutated fluorescent protein sensors, the authors demonstrate dynamic changes in NADPH and the NADH/NAD(+) ratio during photosynthesis and photorespiration at the subcellular level in planta.